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Tide Fluor™ 7WS azide [TF7WS azide]

Tide Fluor™ 7WS (TF7WS) family has the spectral properties similar to those of Cy7, IRDye 800 and Alexa Fluor 750. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF7-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with Cy7, IRDye 800 and Alexa Fluor 750. In pairing with our Tide Quencher™ 7WS (TQ7WS), a variety of FRET peptides and nucleotides can be developed for detecting proteases and molecular beacons with enhanced sensitivity and stability. This TF7WS product is reactive to alkynes, and useful for click chemistry.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Fluor™ 7WS azide [TF7WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM104.271 µL521.355 µL1.043 mL5.214 mL10.427 mL
5 mM20.854 µL104.271 µL208.542 µL1.043 mL2.085 mL
10 mM10.427 µL52.135 µL104.271 µL521.355 µL1.043 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272

References

View all 25 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
Page updated on September 19, 2024

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Catalog Number2304
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Physical properties

Molecular weight

959.04

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.049

Extinction coefficient (cm -1 M -1)

275000

Excitation (nm)

756

Emission (nm)

780

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching&nbsp;fluorophores&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching&nbsp;fluorophores&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching&nbsp;fluorophores&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).