Name: Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
Brand: AAT Bioquest
SKU: 2213
Availability: InStock

Tide Quencher™ 2WS alkyne

TQ2WS alkyne

TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2WS product is reactive to azides, and useful for click chemistry. TQ2WS has much higher water solubility than TQ2. We recommend that you use TQ2WS for the applications that require good water solubility.

Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).

Protocol

COA

Catalog	Size	Price	Quantity
2213	1 mg	Price
2214	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS alkyne [TQ2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.516 µL	947.58 µL	1.895 mL	9.476 mL	18.952 mL
5 mM	37.903 µL	189.516 µL	379.032 µL	1.895 mL	3.79 mL
10 mM	18.952 µL	94.758 µL	189.516 µL	947.58 µL	1.895 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	527.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher

Authors:

Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.

Journal:

Photochem Photobiol Sci (2018): 1739-1748

Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA

Authors:

Li, Y., Sun, L., Zhao, Q.

Journal:

Anal Bioanal Chem (2018): 6269-6277

Forster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing

Authors:

Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.

Journal:

Anal Sci (2017): 137-142

The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs

Authors:

Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.

Journal:

Talanta (2017): 289-294

Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application

Authors:

Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.

Journal:

Cell Med (2015): 57-62

Physical properties

Molecular weight	527.66
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Correction factor (260 nm)	0.100
Correction factor (280 nm)	0.120
Extinction coefficient (cm ^-1 M ^-1)	48000

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet (Catalog 2213)
Safety Data Sheet (Catalog 2214)
Certificate of Analysis

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Page updated on October 23, 2025