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Tide Quencher™ 2WS alkyne [TQ2WS alkyne]

TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2WS product is reactive to azides, and useful for click chemistry. TQ2WS has much higher water solubility than TQ2. We recommend that you use TQ2WS for the applications that require good water solubility.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS alkyne [TQ2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM189.516 µL947.58 µL1.895 mL9.476 mL18.952 mL
5 mM37.903 µL189.516 µL379.032 µL1.895 mL3.79 mL
10 mM18.952 µL94.758 µL189.516 µL947.58 µL1.895 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]9000010.1490.136
Tide Quencher™ 5WS alkyne [TQ5WS alkyne]1300000.0720.082
Tide Quencher™ 6WS alkyne [TQ6WS alkyne]1300000.1200.102
Tide Quencher™ 7WS alkyne [TQ7WS alkyne]1400000.0720.091
Tide Quencher™ 1 alkyne [TQ1 alkyne]200000.1470.194
Tide Quencher™ 2 alkyne [TQ2 alkyne]210000.1000.12
Tide Quencher™ 3 alkyne [TQ3 alkyne]220000.0850.091

Citations

View all 7 citations: Citation Explorer
Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748
Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277
Forster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142
The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294
Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62

References

View all 1 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Page updated on September 18, 2024

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Physical properties

Molecular weight

527.66

Solvent

DMSO

Spectral properties

Absorbance (nm)

541

Correction Factor (260 nm)

0.100

Correction Factor (280 nm)

0.120

Extinction coefficient (cm -1 M -1)

48000

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C); Minimize light exposure
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching dyes&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching dyes&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).
Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching dyes&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).