Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Tide Quencher™ 2WS alkyne [TQ2WS alkyne]

Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Ordering information
Price ()
Catalog Number2213
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight527.66
SolventDMSO
Spectral properties
Absorbance (nm)541
Correction Factor (260 nm)0.100
Correction Factor (280 nm)0.120
Extinction coefficient (cm -1 M -1)48000
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Related products
Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]
Tide Quencher™ 4WS acid [TQ4WS acid]
Tide Quencher™ 4WS amine [TQ4WS amine]
Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*
Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*
Tide Quencher™ 4WS maleimide [TQ4WS maleimide]
Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]
Tide Quencher™ 4WS azide [TQ4WS azide]
Tide Quencher™ 5WS acid [TQ5WS acid]
Tide Quencher™ 5WS amine [TQ5WS amine]
Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å*
Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*
Tide Quencher™ 5WS maleimide [TQ5WS maleimide]
Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]
Tide Quencher™ 6WS acid [TQ6WS acid]
Tide Quencher™ 6WS amine [TQ6WS amine]
Tide Quencher™ 6WS maleimide [TQ6WS maleimide]
Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE]
Tide Quencher™ 6WS azide [TQ6WS azide]
Tide Quencher™ 7WS acid [TQ7WS acid]
Tide Quencher™ 7WS amine [TQ7WS amine]
Tide Quencher™ 7WS maleimide [TQ7WS maleimide]
Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]
Tide Quencher™ 1 azide [TQ1 azide]
Tide Quencher™ 1 acid [TQ1 acid]
Tide Quencher™ 1 amine [TQ1 amine]
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*
Tide Quencher™ 1 maleimide [TQ1 maleimide]
Tide Quencher™ 1 phosphoramidite [TQ1 phosphoramidite]
Tide Quencher™ 1 succinimidyl ester [TQ1 SE]
Tide Quencher™ 2 acid [TQ2 acid]
Tide Quencher™ 2 amine [TQ2 amine]
Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å*
Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å*
Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite]
Tide Quencher™ 2 succinimidyl ester [TQ2 SE]
Tide Quencher™ 2 azide [TQ2 azide]
Tide Quencher™ 3 acid [TQ3 acid]
Tide Quencher™ 3 amine [TQ3 amine]
Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å*
Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*
Tide Quencher™ 3 maleimide [TQ3 maleimide]
Tide Quencher™ 3WS acid [TQ3WS acid]
Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite]
Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE]
Tide Quencher™ 3 succinimidyl ester [TQ3 SE]
Tide Quencher™ 3 azide [TQ3 azide]
Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]
Tide Quencher™ 5WS azide [TQ5WS azide]
Tide Quencher™ 7WS azide [TQ7WS azide]
Show More (51)

OverviewpdfSDSpdfProtocol


Molecular weight
527.66
Absorbance (nm)
541
Correction Factor (260 nm)
0.100
Correction Factor (280 nm)
0.120
Extinction coefficient (cm -1 M -1)
48000
TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2WS product is reactive to azides, and useful for click chemistry. TQ2WS has much higher water solubility than TQ2. We recommend that you use TQ2WS for the applications that require good water solubility.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS alkyne [TQ2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM189.516 µL947.58 µL1.895 mL9.476 mL18.952 mL
5 mM37.903 µL189.516 µL379.032 µL1.895 mL3.79 mL
10 mM18.952 µL94.758 µL189.516 µL947.58 µL1.895 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)541
Correction Factor (260 nm)0.100
Correction Factor (280 nm)0.120
Extinction coefficient (cm -1 M -1)48000

Product family


NameExtinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]9000010.1490.136
Tide Quencher™ 5WS alkyne [TQ5WS alkyne]1300000.0720.082
Tide Quencher™ 6WS alkyne [TQ6WS alkyne]1300000.1200.102
Tide Quencher™ 7WS alkyne [TQ7WS alkyne]1400000.0720.091
Tide Quencher™ 1 alkyne [TQ1 alkyne]200000.1470.194
Tide Quencher™ 2 alkyne [TQ2 alkyne]210000.1000.12
Tide Quencher™ 3 alkyne [TQ3 alkyne]220000.0850.091

Citations


View all 7 citations: Citation Explorer
Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277
Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748
The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294
Forster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142
Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62
Synthesis, in vitro evaluation, and in vivo metabolism of fluor/quencher compounds containing IRDye 800CW and Black Hole Quencher-3 (BHQ-3)
Authors: Linder, K. E., Metcalfe, E., Nanjappan, P., Arunachalam, T., Ramos, K., Skedzielewski, T. M., Marinelli, E. R., Tweedle, M. F., Nunn, A. D., Swenson, R. E.
Journal: Bioconjug Chem (2011): 1287-97
Cy5.5-Gly-Pro-Leu-Gly-Val-Arg-Gly-Cys-Ferritin-black hole quencher-3 nanocages
Authors: Leung, K.
Journal: me="2213.enl" path="C:\Users\aatbi\Dropbox (AAT Bioquest)\Website Working Files\Product References\2213.enl">2213.enlEndNote665L (2004): se name="2213.enl" path="C:\Users\aatbi\Dropb

References


View all 1 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288