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Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2WS product is reactive to azides, and useful for click chemistry. TQ2WS has much higher water solubility than TQ2. We recommend that you use TQ2WS for the applications that require good water solubility.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS alkyne [TQ2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 189.516 µL | 947.58 µL | 1.895 mL | 9.476 mL | 18.952 mL |
| 5 mM | 37.903 µL | 189.516 µL | 379.032 µL | 1.895 mL | 3.79 mL |
| 10 mM | 18.952 µL | 94.758 µL | 189.516 µL | 947.58 µL | 1.895 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Tide Quencher™ 1 alkyne [TQ1 alkyne] | 20000 | 0.147 | 0.194 |
| Tide Quencher™ 2 alkyne [TQ2 alkyne] | 21000 | 0.100 | 0.12 |
| Tide Quencher™ 3 alkyne [TQ3 alkyne] | 22000 | 0.085 | 0.091 |
| Tide Quencher™ 4WS alkyne [TQ4WS alkyne] | 900001 | 0.149 | 0.136 |
| Tide Quencher™ 5WS alkyne [TQ5WS alkyne] | 130000 | 0.072 | 0.082 |
| Tide Quencher™ 6WS alkyne [TQ6WS alkyne] | 130000 | 0.120 | 0.102 |
| Tide Quencher™ 7WS alkyne [TQ7WS alkyne] | 140000 | 0.072 | 0.091 |
Citations
View all 7 citations: Citation Explorer
Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748
Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277
Forster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142
The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294
Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62
References
View all 1 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Page updated on April 22, 2025
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