Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 527.66 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 541 |
Correction Factor (260 nm) | 0.100 |
Correction Factor (280 nm) | 0.120 |
Extinction coefficient (cm -1 M -1) | 48000 |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 2WS maleimide [TQ2WS maleimide] |
Tide Quencher™ 2WS acid [TQ2WS acid] |
Related products
Overview | ![]() ![]() |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), Tide Quencher Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 527.66 | Absorbance (nm) 541 | Correction Factor (260 nm) 0.100 | Correction Factor (280 nm) 0.120 | Extinction coefficient (cm -1 M -1) 48000 |
TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2WS product is reactive to azides, and useful for click chemistry. TQ2WS has much higher water solubility than TQ2. We recommend that you use TQ2WS for the applications that require good water solubility.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS alkyne [TQ2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 189.516 µL | 947.58 µL | 1.895 mL | 9.476 mL | 18.952 mL |
5 mM | 37.903 µL | 189.516 µL | 379.032 µL | 1.895 mL | 3.79 mL |
10 mM | 18.952 µL | 94.758 µL | 189.516 µL | 947.58 µL | 1.895 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 541 |
Correction Factor (260 nm) | 0.100 |
Correction Factor (280 nm) | 0.120 |
Extinction coefficient (cm -1 M -1) | 48000 |
Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS alkyne [TQ4WS alkyne] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS alkyne [TQ5WS alkyne] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS alkyne [TQ6WS alkyne] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS alkyne [TQ7WS alkyne] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 alkyne [TQ1 alkyne] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 alkyne [TQ2 alkyne] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 alkyne [TQ3 alkyne] | 22000 | 0.085 | 0.091 |
Images

Figure 1. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Citations
View all 7 citations: Citation Explorer
Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277
Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748
The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294
Forster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142
Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62
Synthesis, in vitro evaluation, and in vivo metabolism of fluor/quencher compounds containing IRDye 800CW and Black Hole Quencher-3 (BHQ-3)
Authors: Linder, K. E., Metcalfe, E., Nanjappan, P., Arunachalam, T., Ramos, K., Skedzielewski, T. M., Marinelli, E. R., Tweedle, M. F., Nunn, A. D., Swenson, R. E.
Journal: Bioconjug Chem (2011): 1287-97
Authors: Linder, K. E., Metcalfe, E., Nanjappan, P., Arunachalam, T., Ramos, K., Skedzielewski, T. M., Marinelli, E. R., Tweedle, M. F., Nunn, A. D., Swenson, R. E.
Journal: Bioconjug Chem (2011): 1287-97
Cy5.5-Gly-Pro-Leu-Gly-Val-Arg-Gly-Cys-Ferritin-black hole quencher-3 nanocages
Authors: Leung, K.
Journal: me="2213.enl" path="C:\Users\aatbi\Dropbox (AAT Bioquest)\Website Working Files\Product References\2213.enl">2213.enlEndNote665L (2004): se name="2213.enl" path="C:\Users\aatbi\Dropb
Authors: Leung, K.
Journal: me="2213.enl" path="C:\Users\aatbi\Dropbox (AAT Bioquest)\Website Working Files\Product References\2213.enl">2213.enlEndNote665L (2004): se name="2213.enl" path="C:\Users\aatbi\Dropb
References
View all 1 references: Citation Explorer
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288