Tide Quencher™ 2WS alkyne [TQ2WS alkyne]

Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	527.66
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Correction Factor (260 nm)	0.100
Correction Factor (280 nm)	0.120
Extinction coefficient (cm ^-1 M ^-1)	48000

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Tide Quencher™ 2WS maleimide [TQ2WS maleimide]

Tide Quencher™ 2WS acid [TQ2WS acid]

Overview SDS Protocol

Molecular weight

527.66

Absorbance (nm)

541

Correction Factor (260 nm)

0.100

Correction Factor (280 nm)

0.120

Extinction coefficient (cm ^-1 M ^-1)

48000

TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ2WS product is reactive to azides, and useful for click chemistry. TQ2WS has much higher water solubility than TQ2. We recommend that you use TQ2WS for the applications that require good water solubility.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS alkyne [TQ2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.516 µL	947.58 µL	1.895 mL	9.476 mL	18.952 mL
5 mM	37.903 µL	189.516 µL	379.032 µL	1.895 mL	3.79 mL
10 mM	18.952 µL	94.758 µL	189.516 µL	947.58 µL	1.895 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	541
Correction Factor (260 nm)	0.100
Correction Factor (280 nm)	0.120
Extinction coefficient (cm ^-1 M ^-1)	48000

Product Family

Name	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (260 nm)	Correction Factor (280 nm)
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]	90000¹	0.149	0.136
Tide Quencher™ 5WS alkyne [TQ5WS alkyne]	130000	0.072	0.082
Tide Quencher™ 6WS alkyne [TQ6WS alkyne]	130000	0.120	0.102
Tide Quencher™ 7WS alkyne [TQ7WS alkyne]	140000	0.072	0.091
Tide Quencher™ 1 alkyne [TQ1 alkyne]	20000	0.147	0.194
Tide Quencher™ 2 alkyne [TQ2 alkyne]	21000	0.100	0.12
Tide Quencher™ 3 alkyne [TQ3 alkyne]	22000	0.085	0.091

Images

Figure 1. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).

Citations

View all 7 citations: Citation Explorer

Development of aptamer fluorescent switch assay for aflatoxin B1 by using fluorescein-labeled aptamer and black hole quencher 1-labeled complementary DNA
Authors: Li, Y., Sun, L., Zhao, Q.
Journal: Anal Bioanal Chem (2018): 6269-6277

Cyclopeptidic photosensitizer prodrugs as proteolytically triggered drug delivery systems of pheophorbide A: part II - co-loading of pheophorbide A and black hole quencher
Authors: Bouilloux, J., Yuschenko, O., Dereka, B., Boso, G., Babic, A., Zbinden, H., Vauthey, E., Lange, N.
Journal: Photochem Photobiol Sci (2018): 1739-1748

The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs
Authors: Xiang, D., Li, F., Wu, C., Shi, B., Zhai, K.
Journal: Talanta (2017): 289-294

Forster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Anal Sci (2017): 137-142

Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application
Authors: Pillai, S. S., Yukawa, H., Onoshima, D., Biju, V., Baba, Y.
Journal: Cell Med (2015): 57-62

Synthesis, in vitro evaluation, and in vivo metabolism of fluor/quencher compounds containing IRDye 800CW and Black Hole Quencher-3 (BHQ-3)
Authors: Linder, K. E., Metcalfe, E., Nanjappan, P., Arunachalam, T., Ramos, K., Skedzielewski, T. M., Marinelli, E. R., Tweedle, M. F., Nunn, A. D., Swenson, R. E.
Journal: Bioconjug Chem (2011): 1287-97

Cy5.5-Gly-Pro-Leu-Gly-Val-Arg-Gly-Cys-Ferritin-black hole quencher-3 nanocages
Authors: Leung, K.
Journal: me="2213.enl" path="C:\Users\aatbi\Dropbox (AAT Bioquest)\Website Working Files\Product References\2213.enl">2213.enlEndNote665L (2004): se name="2213.enl" path="C:\Users\aatbi\Dropb

References

View all 1 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288