logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Tide Quencher™ 4WS alkyne
TQ4WS alkyne
TQ4WS is designed to be a superior quencher to ROX, TF4, iFluor® 594, Alexa Fluor® 594 and Texas Red®. TQ4WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ4WS-DBCO product is reactive to azides under copper-free conditions, and useful for click chemistry. DBCO is probably the most common alkyne among the strain promoted alkyne-azide cycloaddition (SPAAC), which is also termed as the Cu-free click reaction. Cyclooctynes and azides exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as amines. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. DBCO (dibenzocyclooctynes) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with amines or hydroxyls that are naturally present in many biomolecules. Additionally, reaction of the DBCO group with the azide group is significantly faster than with sulfhydryl groups (–SH, thiol).
<p>Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching dyes&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).</p>
<p>Click chemistry is a method for attaching a&nbsp;probe&nbsp;or&nbsp;substrate&nbsp;of interest to a specific biomolecule, a process called&nbsp;bioconjugation. The possibility of attaching dyes&nbsp;and other&nbsp;reporter molecules&nbsp;has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an&nbsp;azide&nbsp;with an&nbsp;alkyne&nbsp;to form a 5-membered&nbsp;heteroatom&nbsp;ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne&nbsp;Cycloaddition&nbsp;(CuAAC).</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20691 mg
Price
 
Physical properties
Molecular weight1158.49
SolventDMSO
Spectral properties
Absorbance (nm)605
Correction factor (260 nm)0.149
Correction factor (280 nm)0.136
Extinction coefficient (cm -1 M -1)
90000
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 13, 2025