Name: Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]
Brand: AAT Bioquest
SKU: 2058
Price: 232 USD
Availability: InStock

Tide Quencher™ 2WS succinimidyl ester

TQ2WS, SE

TQ2WS is designed to be a superior quencher to FAM, HEX, TET, JOE, TF2 and rhodamine 6G. TQ2WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with much greater water solubility than other existing quenchers for labeling oligonucleotides and peptides. This TQ2WS product is the most-water soluble and non-fluorescent quencher for labeling oligos and peptides. We have demonstrated that TQ2WS is extremely useful for enhancing the water solubility of hydrophobic peptides. TQ2WS, SE readily reacts with aliphatic amines including amino acids, peptides, amino-modified oligos and proteins.

<p>Dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2058	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	145.167 µL	725.837 µL	1.452 mL	7.258 mL	14.517 mL
5 mM	29.033 µL	145.167 µL	290.335 µL	1.452 mL	2.903 mL
10 mM	14.517 µL	72.584 µL	145.167 µL	725.837 µL	1.452 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	688.86 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Conjugation Chemistry Markedly Impacts Toxicity and Biodistribution of Targeted Nanoparticles, Mediated by Complement Activation

Authors:

Zaleski, Michael H and Chase, Liam S and Hood, Elizabeth D and Wang, Zhicheng and Nong, Jia and Espy, Carolann L and Zamora, Marco E and Wu, Jichuan and Morrell, Lianne J and Muzykantov, Vladimir R and others,

Journal:

Advanced Materials (2024): 2409945

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation

Authors:

Perlenfein, Tyler J and Murphy, Regina M

Journal:

Journal of Biological Chemistry (2017): jbc--M117

Real-Time Detection of a Self-Replicating RNA Enzyme

Authors:

Olea, Charles and Joyce, Gerald F

Journal:

Molecules (2016): 1310

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia

Authors:

Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G

Journal:

American journal of obstetrics and gynecology (2015): 82--e1

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces

Authors:

Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined

Journal:

ECS Transactions (2015): 9--16

References

View all 25 references: Citation Explorer

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens

Authors:

Yang GP, Erdman DD, Tondella ML, Fields BS.

Journal:

J Virol Methods (2009): 288

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe

Authors:

Andreoni A, Bondani M, Nardo L.

Journal:

Photochem Photobiol Sci (2009): 1202

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer

Authors:

Ogawa M, Kosaka N, Choyke PL, Kobayashi H.

Journal:

Bioconjug Chem (2009): 147

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates

Authors:

Kim GI, Kim KW, Oh MK, Sung YM.

Journal:

Nanotechnology (2009): 175503

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening

Authors:

Yu X, Sainz B, Jr., Uprichard SL.

Journal:

Antimicrob Agents Chemother (2009): 4311

Physical properties

Molecular weight	688.86
Solvent	DMSO

Spectral properties

Absorbance (nm)	541
Correction factor (260 nm)	0.100
Correction factor (280 nm)	0.120
Extinction coefficient (cm ^-1 M ^-1)	48000

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Fax
Email	sales@aatbio.com
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Custom size	Inquire
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025