Tide Quencher™ 5.1WS acid [TQ5.1WS acid]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 952.00 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 5.1WS amine [TQ5.1WS amine] |
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide] |
Tide Quencher™ 5.1WS azide [TQ5.1WS azide] |
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 952.00 |
Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS acid is primarily used for labeling amino-modified oligonucleotides and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5.1WS acid [TQ5.1WS acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 105.042 µL | 525.21 µL | 1.05 mL | 5.252 mL | 10.504 mL |
5 mM | 21.008 µL | 105.042 µL | 210.084 µL | 1.05 mL | 2.101 mL |
10 mM | 10.504 µL | 52.521 µL | 105.042 µL | 525.21 µL | 1.05 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 50 references: Citation Explorer
Evaluation of probe-based ultra-sensitive detection of miRNA using a single-molecule fluorescence imaging method: miR-126 used as the model.
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Authors: Liu, Longkai and Wang, Xiaoning and Li, Yan and Liu, Jianwei
Journal: Frontiers in bioengineering and biotechnology (2023): 1081488
Synthesis of Multifunctional Polymersomes Prepared by Polymerization-Induced Self-Assembly.
Authors: Phan, Hien and Cavanagh, Robert and Jacob, Philippa and Destouches, Damien and Vacherot, Francis and Brugnoli, Benedetta and Howdle, Steve and Taresco, Vincenzo and Couturaud, Benoit
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Authors: Phan, Hien and Cavanagh, Robert and Jacob, Philippa and Destouches, Damien and Vacherot, Francis and Brugnoli, Benedetta and Howdle, Steve and Taresco, Vincenzo and Couturaud, Benoit
Journal: Polymers (2023)
Tuning the Polarity of Antibiotic-Cy5 Conjugates Enables Highly Selective Labeling of Binding Sites.
Authors: Graßl, Fabian and Konrad, Maike M B and Krüll, Jasmin and Pezerovic, Azra and Zähnle, Leon and Burkovski, Andreas and Heinrich, Markus R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202301208
Authors: Graßl, Fabian and Konrad, Maike M B and Krüll, Jasmin and Pezerovic, Azra and Zähnle, Leon and Burkovski, Andreas and Heinrich, Markus R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202301208
Highly sensitive and selective antibody microarrays based on a Cy5-antibody complexes coupling ES-biochip for E. coli and Salmonella detection.
Authors: Hormsombut, Timpika and Rijiravanich, Patsamon and Surareungchai, Werasak and Kalasin, Surachate
Journal: RSC advances (2022): 24760-24768
Authors: Hormsombut, Timpika and Rijiravanich, Patsamon and Surareungchai, Werasak and Kalasin, Surachate
Journal: RSC advances (2022): 24760-24768
Repurposing an atherosclerosis targeting peptide for tumor imaging.
Authors: Kovacs, Luciana and Davis, Ryan A and Ganguly, Tanushree and Chammas, Roger and Sutcliffe, Julie L
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2022): 112469
Authors: Kovacs, Luciana and Davis, Ryan A and Ganguly, Tanushree and Chammas, Roger and Sutcliffe, Julie L
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2022): 112469
Incoherent broadband cavity-enhanced absorption spectroscopy for sensitive measurement of nutrients and microalgae.
Authors: Zhang, Haodong and Luo, Jing and Hou, Saimei and Xu, Zhanpeng and Evans, Julian and He, Sailing
Journal: Applied optics (2022): 3400-3408
Authors: Zhang, Haodong and Luo, Jing and Hou, Saimei and Xu, Zhanpeng and Evans, Julian and He, Sailing
Journal: Applied optics (2022): 3400-3408
Small molecule based EGFR targeting of biodegradable nanoparticles containing temozolomide and Cy5 dye for greatly enhanced image-guided glioblastoma therapy.
Authors: Schmitt, Rebecca R and Mahajan, Supriya D and Pliss, Artem and Prasad, Paras N
Journal: Nanomedicine : nanotechnology, biology, and medicine (2022): 102513
Authors: Schmitt, Rebecca R and Mahajan, Supriya D and Pliss, Artem and Prasad, Paras N
Journal: Nanomedicine : nanotechnology, biology, and medicine (2022): 102513
Water-powered self-propelled magnetic nanobot for rapid and highly efficient capture of circulating tumor cells.
Authors: Wavhale, Ravindra D and Dhobale, Kshama D and Rahane, Chinmay S and Chate, Govind P and Tawade, Bhausaheb V and Patil, Yuvraj N and Gawade, Sandesh S and Banerjee, Shashwat S
Journal: Communications chemistry (2021): 159
Authors: Wavhale, Ravindra D and Dhobale, Kshama D and Rahane, Chinmay S and Chate, Govind P and Tawade, Bhausaheb V and Patil, Yuvraj N and Gawade, Sandesh S and Banerjee, Shashwat S
Journal: Communications chemistry (2021): 159
Nucleic Acid-Gated Covalent Organic Frameworks for Cancer-Specific Imaging and Drug Release.
Authors: Gao, Peng and Shen, Xiaoying and Liu, Xiaohan and Chen, Yuanyuan and Pan, Wei and Li, Na and Tang, Bo
Journal: Analytical chemistry (2021): 11751-11757
Authors: Gao, Peng and Shen, Xiaoying and Liu, Xiaohan and Chen, Yuanyuan and Pan, Wei and Li, Na and Tang, Bo
Journal: Analytical chemistry (2021): 11751-11757
Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.
Authors: Abe, Masahiro and Nishihara, Ryo and Kim, Sung-Bae and Suzuki, Koji
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 103-110
Authors: Abe, Masahiro and Nishihara, Ryo and Kim, Sung-Bae and Suzuki, Koji
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 103-110