Tide Quencher™ 5.1WS amine [TQ5.1WS amine]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1108.11 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide] |
Tide Quencher™ 5.1WS acid [TQ5.1WS acid] |
Tide Quencher™ 5.1WS azide [TQ5.1WS azide] |
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne] |
Related products
Overview | SDSProtocol |
See also: Tide Quencher Dyes
Molecular weight 1108.11 |
Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS amine is primarily used for labeling biomolecules that contain a carbonyl group. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5.1WS amine [TQ5.1WS amine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 90.244 µL | 451.219 µL | 902.437 µL | 4.512 mL | 9.024 mL |
5 mM | 18.049 µL | 90.244 µL | 180.487 µL | 902.437 µL | 1.805 mL |
10 mM | 9.024 µL | 45.122 µL | 90.244 µL | 451.219 µL | 902.437 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). Tide Quencher™ 5.1WS amine is primarily used for labeling biomolecules that contain a carbonyl group.
References
View all 50 references: Citation Explorer
Development of Porcine Monoclonal Antibodies with In Vitro Neutralizing Activity against Classical Swine Fever Virus from C-Strain E2-Specific Single B Cells.
Authors: Wang, Lihua and Madera, Rachel and Li, Yuzhen and Gladue, Douglas P and Borca, Manuel V and McIntosh, Michael T and Shi, Jishu
Journal: Viruses (2023)
Authors: Wang, Lihua and Madera, Rachel and Li, Yuzhen and Gladue, Douglas P and Borca, Manuel V and McIntosh, Michael T and Shi, Jishu
Journal: Viruses (2023)
Chemogenetic inhibition of TrkB signalling reduces phrenic motor neuron survival and size.
Authors: Fogarty, Matthew J and Dasgupta, Debanjali and Khurram, Obaid U and Sieck, Gary C
Journal: Molecular and cellular neurosciences (2023): 103847
Authors: Fogarty, Matthew J and Dasgupta, Debanjali and Khurram, Obaid U and Sieck, Gary C
Journal: Molecular and cellular neurosciences (2023): 103847
Synthesis of Short Peptides with Perfluoroalkyl Side Chains and Evaluation of Their Cellular Uptake Efficiency.
Authors: Kadota, Koji and Mikami, Toshiki and Kohata, Ai and Morimoto, Jumpei and Sando, Shinsuke and Aikawa, Kohsuke and Okazoe, Takashi
Journal: Chembiochem : a European journal of chemical biology (2023): e202300374
Authors: Kadota, Koji and Mikami, Toshiki and Kohata, Ai and Morimoto, Jumpei and Sando, Shinsuke and Aikawa, Kohsuke and Okazoe, Takashi
Journal: Chembiochem : a European journal of chemical biology (2023): e202300374
AxonQuantifier: A semi-automated program for quantifying axonal density from whole-mounted optic nerves.
Authors: Peng, Micalla G and Lee, Jonathan and Ho, Wilson and Kim, Timothy and Yao, Petcy and Medvidovic, Sasha and Alas, Basheer and Wu, Vivian and Runner, Margaret M and Gokoffski, Kimberly K
Journal: Journal of neuroscience methods (2023): 109895
Authors: Peng, Micalla G and Lee, Jonathan and Ho, Wilson and Kim, Timothy and Yao, Petcy and Medvidovic, Sasha and Alas, Basheer and Wu, Vivian and Runner, Margaret M and Gokoffski, Kimberly K
Journal: Journal of neuroscience methods (2023): 109895
Flow Cytometric Identification and Enumeration of Monocyte Subsets in Bovine and Water Buffalo Peripheral Blood.
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600.
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Mailuoning oral liquid attenuates convalescent cerebral ischemia by inhibiting AMPK/mTOR-associated apoptosis and promoting CREB/BDNF-mediated neuroprotection.
Authors: Liu, Xiaoqiong and Fan, Lingling and Li, Jian and Bai, Ziyu and Wang, Yue and Liu, Yafang and Jiang, Hong and Tao, Anhua and Li, Xiang and Zhang, Hui and Tan, Ninghua
Journal: Journal of ethnopharmacology (2023): 116731
Authors: Liu, Xiaoqiong and Fan, Lingling and Li, Jian and Bai, Ziyu and Wang, Yue and Liu, Yafang and Jiang, Hong and Tao, Anhua and Li, Xiang and Zhang, Hui and Tan, Ninghua
Journal: Journal of ethnopharmacology (2023): 116731
Evaluation of Slowfade Diamond as a buffer for STORM microscopy.
Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558
Authors: Boukhatem, Hadjer and Durel, Beatrice and Raimbault, Manon and Laurent, Audrey and Olivier, Nicolas
Journal: Biomedical optics express (2023): 550-558
Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Click-Functionalization of Silanized Carbon Nanotubes: From Inorganic Heterostructures to Biosensing Nanohybrids.
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)