Tide Quencher™ 5.1WS azide [TQ5.1WS azide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1020.08 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 5.1WS amine [TQ5.1WS amine] |
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide] |
Tide Quencher™ 5.1WS acid [TQ5.1WS acid] |
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1020.08 |
Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS Azide is an excellent building block for preparing TQ5.1WS-labeled probes from alkyne-modified oligonucleotides (including DBCO-modified oligonucleotides) and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5.1WS azide [TQ5.1WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.032 µL | 490.158 µL | 980.315 µL | 4.902 mL | 9.803 mL |
5 mM | 19.606 µL | 98.032 µL | 196.063 µL | 980.315 µL | 1.961 mL |
10 mM | 9.803 µL | 49.016 µL | 98.032 µL | 490.158 µL | 980.315 µL |
Molarity calculator
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References
View all 50 references: Citation Explorer
Fluorescent Properties of Cyanine Dyes As a Matter of the Environment.
Authors: Fan, Fan and Povedailo, Vladimir A and Lysenko, Ivan L and Seviarynchyk, Tatsiana P and Sharko, Olga L and Mazunin, Ilya O and Shmanai, Vadim V
Journal: Journal of fluorescence (2023)
Authors: Fan, Fan and Povedailo, Vladimir A and Lysenko, Ivan L and Seviarynchyk, Tatsiana P and Sharko, Olga L and Mazunin, Ilya O and Shmanai, Vadim V
Journal: Journal of fluorescence (2023)
DNA-Programmed Lipid Nanoreactors for Synthesis of Carbohydrate Mimetics by Fusion of Aqueous Sub-attoliter Compartments.
Authors: Tian, Xinwei and Risgaard, Nikolaj Alexander and Löffler, Philipp M G and Vogel, Stefan
Journal: Journal of the American Chemical Society (2023)
Authors: Tian, Xinwei and Risgaard, Nikolaj Alexander and Löffler, Philipp M G and Vogel, Stefan
Journal: Journal of the American Chemical Society (2023)
Lactose-modified enzyme-sensitive branched polymers as a nanoscale liver cancer-targeting MRI contrast agent.
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Journal: Nanoscale (2023): 809-819
Enhanced imaging of protein-specific palmitoylation with HCR-based cis-membrane multi-FRET.
Authors: Fu, Yixin and Qian, Husun and Yang, Yujun and Li, Junjie and Xie, Guoming
Journal: Talanta (2023): 124972
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Journal: Talanta (2023): 124972
Granzyme B Turns Nanoparticle Fluorescence "On" for Imaging Cytotoxic T Lymphocyte Activity in Vivo.
Authors: Xu, Lingling and Liu, Nanhui and Zhan, Wenjun and Deng, Yu and Chen, Zhaoxia and Liu, Xiaoyang and Gao, Ge and Chen, Qian and Liu, Zhuang and Liang, Gaolin
Journal: ACS nano (2022): 19328-19334
Authors: Xu, Lingling and Liu, Nanhui and Zhan, Wenjun and Deng, Yu and Chen, Zhaoxia and Liu, Xiaoyang and Gao, Ge and Chen, Qian and Liu, Zhuang and Liang, Gaolin
Journal: ACS nano (2022): 19328-19334
Dendrimer-2PMPA selectively blocks upregulated microglial GCPII activity and improves cognition in a mouse model of multiple sclerosis.
Authors: Hollinger, Kristen R and Sharma, Anjali and Tallon, Carolyn and Lovell, Lyndah and Thomas, Ajit G and Zhu, Xiaolei and Wiseman, Robyn and Wu, Ying and Kambhampati, Siva P and Liaw, Kevin and Sharma, Rishi and Rojas, Camilo and Rais, Rana and Kannan, Sujatha and Kannan, Rangaramanujam M and Slusher, Barbara S
Journal: Nanotheranostics (2022): 126-142
Authors: Hollinger, Kristen R and Sharma, Anjali and Tallon, Carolyn and Lovell, Lyndah and Thomas, Ajit G and Zhu, Xiaolei and Wiseman, Robyn and Wu, Ying and Kambhampati, Siva P and Liaw, Kevin and Sharma, Rishi and Rojas, Camilo and Rais, Rana and Kannan, Sujatha and Kannan, Rangaramanujam M and Slusher, Barbara S
Journal: Nanotheranostics (2022): 126-142
ROS Turn Nanoparticle Fluorescence on for Imaging Staphylococcus aureus Infection In Vivo.
Authors: Xu, Lingling and Zhan, Wenjun and Deng, Yu and Liu, Xiaoyang and Gao, Ge and Sun, Xianbao and Liang, Gaolin
Journal: Advanced healthcare materials (2022): e2200453
Authors: Xu, Lingling and Zhan, Wenjun and Deng, Yu and Liu, Xiaoyang and Gao, Ge and Sun, Xianbao and Liang, Gaolin
Journal: Advanced healthcare materials (2022): e2200453
Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.
Authors: Gebhard, Julian and Hirsch, Lara and Schwechheimer, Christian and Wagenknecht, Hans-Achim
Journal: Bioconjugate chemistry (2022): 1634-1642
Authors: Gebhard, Julian and Hirsch, Lara and Schwechheimer, Christian and Wagenknecht, Hans-Achim
Journal: Bioconjugate chemistry (2022): 1634-1642
Orthogonal Covalent Entrapment of Cargo into Biodegradable Polymeric Micelles via Native Chemical Ligation.
Authors: Hebels, Erik R and Bindt, Felix and Walther, Johanna and van Geijn, Michiel and Weterings, Jimmy and Hu, Qizhi and Colombo, Claudio and Liskamp, Rob and Rijcken, Cristianne and Hennink, Wim E and Vermonden, Tina
Journal: Biomacromolecules (2022)
Authors: Hebels, Erik R and Bindt, Felix and Walther, Johanna and van Geijn, Michiel and Weterings, Jimmy and Hu, Qizhi and Colombo, Claudio and Liskamp, Rob and Rijcken, Cristianne and Hennink, Wim E and Vermonden, Tina
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Chemical Synthesis of a Functional Fluorescent-Tagged α-Bungarotoxin.
Authors: Brun, Oliver and Zoukimian, Claude and Oliveira-Mendes, Barbara and Montnach, Jérôme and Lauzier, Benjamin and Ronjat, Michel and Béroud, Rémy and Lesage, Frédéric and Boturyn, Didier and De Waard, Michel
Journal: Toxins (2022)
Authors: Brun, Oliver and Zoukimian, Claude and Oliveira-Mendes, Barbara and Montnach, Jérôme and Lauzier, Benjamin and Ronjat, Michel and Béroud, Rémy and Lesage, Frédéric and Boturyn, Didier and De Waard, Michel
Journal: Toxins (2022)