Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1074.12 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 5.1WS amine [TQ5.1WS amine] |
Tide Quencher™ 5.1WS acid [TQ5.1WS acid] |
Tide Quencher™ 5.1WS azide [TQ5.1WS azide] |
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1074.12 |
Tide Quencher™ 5.1WS (TQ5.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy5, Cy5.5, Alexa Fluor® 647, Alexa Fluor® 647, iFluor® 647 and iFluor® 680. It is an improved version of TQ5 and QSY 21 and BHQ3). TQ5.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency. Tide Quencher™ 5.1WS maleimide is primarily used for the post-labeling of thiol-modified oligonucleotides and the Cys residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ5.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ5.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 93.099 µL | 465.497 µL | 930.995 µL | 4.655 mL | 9.31 mL |
5 mM | 18.62 µL | 93.099 µL | 186.199 µL | 930.995 µL | 1.862 mL |
10 mM | 9.31 µL | 46.55 µL | 93.099 µL | 465.497 µL | 930.995 µL |
Molarity calculator
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References
View all 31 references: Citation Explorer
Design of the New Closo-Dodecarborate-Containing Gemcitabine Analogue for the Albumin-Based Theranostics Composition.
Authors: Raskolupova, Valeria I and Wang, Meiling and Dymova, Maya A and Petrov, Gleb O and Shchudlo, Ivan M and Taskaev, Sergey Yu and Abramova, Tatyana V and Godovikova, Tatyana S and Silnikov, Vladimir N and Popova, Tatyana V
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Raskolupova, Valeria I and Wang, Meiling and Dymova, Maya A and Petrov, Gleb O and Shchudlo, Ivan M and Taskaev, Sergey Yu and Abramova, Tatyana V and Godovikova, Tatyana S and Silnikov, Vladimir N and Popova, Tatyana V
Journal: Molecules (Basel, Switzerland) (2023)
Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734
Development and Characterization of a Novel Peptide-Drug Conjugate with DM1 for Treatment of FGFR2-Positive Tumors.
Authors: Wang, Yayu and Li, Yadan and Cao, Jieqiong and Meng, Qilin and Li, Xiaocen and Zhang, Yibo and Lam, Kit S and Hong, An and Liu, Ruiwu and Chen, Xiaojia
Journal: Biomedicines (2021)
Authors: Wang, Yayu and Li, Yadan and Cao, Jieqiong and Meng, Qilin and Li, Xiaocen and Zhang, Yibo and Lam, Kit S and Hong, An and Liu, Ruiwu and Chen, Xiaojia
Journal: Biomedicines (2021)
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Cyclic RGD-Functionalized closo-Dodecaborate Albumin Conjugates as Integrin Targeting Boron Carriers for Neutron Capture Therapy.
Authors: Kawai, Kazuki and Nishimura, Kai and Okada, Satoshi and Sato, Shinichi and Suzuki, Minoru and Takata, Takushi and Nakamura, Hiroyuki
Journal: Molecular pharmaceutics (2020): 3740-3747
Authors: Kawai, Kazuki and Nishimura, Kai and Okada, Satoshi and Sato, Shinichi and Suzuki, Minoru and Takata, Takushi and Nakamura, Hiroyuki
Journal: Molecular pharmaceutics (2020): 3740-3747
Virus-mimicking mesoporous organosilica nanocapsules with soft framework and rough surface for enhanced cellular uptake and tumor penetration.
Authors: Tao, Jun and Chen, Kun and Su, Xiaodan and Ren, Lili and Zhang, Junjie and Bao, Lei and Dong, Heng and Lu, Guangming and Teng, Zhaogang and Wang, Lianhui
Journal: Biomaterials science (2020): 2227-2233
Authors: Tao, Jun and Chen, Kun and Su, Xiaodan and Ren, Lili and Zhang, Junjie and Bao, Lei and Dong, Heng and Lu, Guangming and Teng, Zhaogang and Wang, Lianhui
Journal: Biomaterials science (2020): 2227-2233
Development of sesbania mosaic virus nanoparticles for imaging.
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507
Authors: Vishnu Vardhan, G P and Hema, M and Sushmitha, C and Savithri, H S and Natraj, Usha and Murthy, M R N
Journal: Archives of virology (2019): 497-507
A Comparative Study on Albumin-Binding Molecules for Targeted Tumor Delivery through Covalent and Noncovalent Approach.
Authors: Um, Wooram and Park, Jooho and Youn, Ahye and Cho, Hanhee and Lim, Seungho and Lee, Jong Won and Yoon, Hong Yeol and Lim, Dong-Kwon and Park, Jae Hyung and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2019): 3107-3118
Authors: Um, Wooram and Park, Jooho and Youn, Ahye and Cho, Hanhee and Lim, Seungho and Lee, Jong Won and Yoon, Hong Yeol and Lim, Dong-Kwon and Park, Jae Hyung and Kim, Kwangmeyung
Journal: Bioconjugate chemistry (2019): 3107-3118
Facile preparation of near-infrared fluorescence and magnetic resonance dual-modality imaging probes based on mesoporous organosilica nanoparticles.
Authors: Li, Yanjiao and Guo, Wenwen and Su, Xiaodan and Lu, Nan and Wu, Guangyao and Ou-Yang, Lin and Dang, Meng and Tao, Jun and Teng, Zhaogang
Journal: Journal of colloid and interface science (2019): 277-286
Authors: Li, Yanjiao and Guo, Wenwen and Su, Xiaodan and Lu, Nan and Wu, Guangyao and Ou-Yang, Lin and Dang, Meng and Tao, Jun and Teng, Zhaogang
Journal: Journal of colloid and interface science (2019): 277-286
Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods.
Authors: Chen, Chun Chieh and Stark, Marie and Baikoghli, Mo and Cheng, R Holland
Journal: Journal of visualized experiments : JoVE (2018)
Authors: Chen, Chun Chieh and Stark, Marie and Baikoghli, Mo and Cheng, R Holland
Journal: Journal of visualized experiments : JoVE (2018)