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Tide Quencher™ 5WS acid [TQ5WS acid]

Dye quencher acids are the common tools for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid through their amino groups. They are the excellent building blocks for developing FRET assays.
Dye quencher acids are the common tools for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid through their amino groups. They are the excellent building blocks for developing FRET assays.
Ordering information
Price ()
Catalog Number2075
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight890.00
SolventDMSO
Spectral properties
Absorbance (nm)661
Correction Factor (260 nm)0.072
Correction Factor (280 nm)0.082
Extinction coefficient (cm -1 M -1)130000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]
Tide Quencher™ 2WS maleimide [TQ2WS maleimide]
Tide Quencher™ 4WS amine [TQ4WS amine]
Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*
Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*
Tide Quencher™ 4WS maleimide [TQ4WS maleimide]
Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]
Tide Quencher™ 4WS azide [TQ4WS azide]
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]
Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å*
Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*
Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]
Tide Quencher™ 6WS amine [TQ6WS amine]
Tide Quencher™ 6WS maleimide [TQ6WS maleimide]
Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE]
Tide Quencher™ 6WS azide [TQ6WS azide]
Tide Quencher™ 6WS alkyne [TQ6WS alkyne]
Tide Quencher™ 7WS amine [TQ7WS amine]
Tide Quencher™ 7WS maleimide [TQ7WS maleimide]
Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]
Tide Quencher™ 7WS alkyne [TQ7WS alkyne]
Tide Quencher™ 1 azide [TQ1 azide]
Tide Quencher™ 1 alkyne [TQ1 alkyne]
Tide Quencher™ 1 amine [TQ1 amine]
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*
Tide Quencher™ 1 maleimide [TQ1 maleimide]
Tide Quencher™ 1 phosphoramidite [TQ1 phosphoramidite]
Tide Quencher™ 1 succinimidyl ester [TQ1 SE]
Tide Quencher™ 2 amine [TQ2 amine]
Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å*
Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å*
Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite]
Tide Quencher™ 2 succinimidyl ester [TQ2 SE]
Tide Quencher™ 2 azide [TQ2 azide]
Tide Quencher™ 2 alkyne [TQ2 alkyne]
Tide Quencher™ 3 amine [TQ3 amine]
Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å*
Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*
Tide Quencher™ 3 maleimide [TQ3 maleimide]
Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite]
Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE]
Tide Quencher™ 3 succinimidyl ester [TQ3 SE]
Tide Quencher™ 3 azide [TQ3 azide]
Tide Quencher™ 3 alkyne [TQ3 alkyne]
Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]
Tide Quencher™ 7WS azide [TQ7WS azide]
Show More (48)

OverviewpdfSDSpdfProtocol


Molecular weight
890.00
Absorbance (nm)
661
Correction Factor (260 nm)
0.072
Correction Factor (280 nm)
0.082
Extinction coefficient (cm -1 M -1)
130000
TQ5WS is designed to be a superior quencher to Cy5, TF5 and Cy5.5. TQ5WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ5WS product is primarily used for labeling peptides.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 5WS acid [TQ5WS acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM112.36 µL561.798 µL1.124 mL5.618 mL11.236 mL
5 mM22.472 µL112.36 µL224.719 µL1.124 mL2.247 mL
10 mM11.236 µL56.18 µL112.36 µL561.798 µL1.124 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Absorbance (nm)661
Correction Factor (260 nm)0.072
Correction Factor (280 nm)0.082
Extinction coefficient (cm -1 M -1)130000

Product family


NameExtinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
Tide Quencher™ 2WS acid [TQ2WS acid]480000.1000.120
Tide Quencher™ 4WS acid [TQ4WS acid]9000010.1490.136
Tide Quencher™ 6WS acid [TQ6WS acid]1300000.1200.102
Tide Quencher™ 7WS acid [TQ7WS acid]1400000.0720.091
Tide Quencher™ 1 acid [TQ1 acid]200000.1470.194
Tide Quencher™ 2 acid [TQ2 acid]210000.1000.12
Tide Quencher™ 3 acid [TQ3 acid]220000.0850.091
Tide Quencher™ 3WS acid [TQ3WS acid]900000.1860.205
Tide Fluor™ 5WS acid [TF5WS acid] *Superior replacement for Cy5*250000-0.027

Citations


View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro

References


View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519