Tide Quencher™ 6WS azide [TQ6WS azide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1007.11 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 694 |
Correction Factor (260 nm) | 0.120 |
Correction Factor (280 nm) | 0.102 |
Extinction coefficient (cm -1 M -1) | 130000 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Alternative formats
Tide Quencher™ 6WS amine [TQ6WS amine] |
Tide Quencher™ 6WS maleimide [TQ6WS maleimide] |
Tide Quencher™ 6WS acid [TQ6WS acid] |
Tide Quencher™ 6WS alkyne [TQ6WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), Tide Quencher Dyes, PCR Detection of Viral DNA/RNA
Molecular weight 1007.11 | Absorbance (nm) 694 | Correction Factor (260 nm) 0.120 | Correction Factor (280 nm) 0.102 | Extinction coefficient (cm -1 M -1) 130000 |
TQ6WS is designed to be a superior quencher to Cy5.5, TF6 and Alexa Fluor 680. TQ6WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQ6WS product is reactive to alkynes, and useful for click chemistry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 6WS azide [TQ6WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 99.294 µL | 496.47 µL | 992.94 µL | 4.965 mL | 9.929 mL |
5 mM | 19.859 µL | 99.294 µL | 198.588 µL | 992.94 µL | 1.986 mL |
10 mM | 9.929 µL | 49.647 µL | 99.294 µL | 496.47 µL | 992.94 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 694 |
Correction Factor (260 nm) | 0.120 |
Correction Factor (280 nm) | 0.102 |
Extinction coefficient (cm -1 M -1) | 130000 |
Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS azide [TQ4WS azide] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 1 azide [TQ1 azide] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 azide [TQ2 azide] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 azide [TQ3 azide] | 22000 | 0.085 | 0.091 |
Tide Quencher™ 5WS azide [TQ5WS azide] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 7WS azide [TQ7WS azide] | 140000 | 0.072 | 0.091 |
Tide Fluor™ 6WS azide [TF6WS azide] | 220000 | - | 0.101 |
Images

Figure 1. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching dyes and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Citations
View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro
References
View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
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Journal: Antimicrob Agents Chemother (2009): 4311
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
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Journal: Bioconjug Chem (2009): 702
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
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Rapid detection and quantification of Propionibacteriaceae
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Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
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Journal: J Virol Methods (2009): 288
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519