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Tide Quencher™ 7.1 CPG [TQ7.1 CPG] *1000 Å*

Tide Quencher™ 7.1 (TQ7.1) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.1 is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.1 CPG is an excellent building block for preparing TQ7.1-labeled oligonucleotides. The oligo prepared from (TQ7.1 CPG) may be deprotected in 0.05M potassium carbonate in methanol for 4 hours at room temperature for 2 hours. Alternatively, the oligo may be deprotected in ammonium hydroxide at room temperature for 24-36 hours. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.1 quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified.

References

View all 16 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
Stepwise Energy Transfer: Near-Infrared Persistent Luminescence from Doped Polymeric Systems.
Authors: Lin, Faxu and Wang, Haiyang and Cao, Yifeng and Yu, Rujun and Liang, Guodong and Huang, Huahua and Mu, Yingxiao and Yang, Zhiyong and Chi, Zhenguo
Journal: Advanced materials (Deerfield Beach, Fla.) (2022): e2108333
Fluorescent energy transfer causing misleading signal in multicolor flow cytometry.
Authors: Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
Single-Molecule FRET Detection of Sub-Nanometer Distance Changes in the Range below a 3-Nanometer Scale.
Authors: Son, Heyjin and Mo, Woori and Park, Jaeil and Lee, Joong-Wook and Lee, Sanghwa
Journal: Biosensors (2020)
PI3KC3 complex subunit NRBF2 is required for apoptotic cell clearance to restrict intestinal inflammation.
Authors: Wu, Ming-Yue and Liu, Le and Wang, Er-Jin and Xiao, Hai-Tao and Cai, Cui-Zan and Wang, Jing and Su, Huanxing and Wang, Yitao and Tan, Jieqiong and Zhang, Zhuohua and Wang, Juan and Yao, Maojing and Ouyang, De-Fang and Yue, Zhenyu and Li, Min and Chen, Ye and Bian, Zhao-Xiang and Lu, Jia-Hong
Journal: Autophagy (2020): 1-16
Page updated on October 11, 2024

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Physical properties

Solvent

MeCN

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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