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Tide Quencher™ 7.1 CPG [TQ7.1 CPG] *500 Å*

Tide Quencher™ 7.1 (TQ7.1) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.1 is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.1 CPG is an excellent building block for preparing TQ7.1-labeled oligonucleotides. The oligo prepared from (TQ7.1 CPG) may be deprotected in 0.05M potassium carbonate in methanol for 4 hours at room temperature for 2 hours. Alternatively, the oligo may be deprotected in ammonium hydroxide at room temperature for 24-36 hours. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.1 quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified.

Spectrum

References

View all 17 references: Citation Explorer
Cooperatively enhanced photothermal-chemotherapy via simultaneously downregulating HSPs and promoting DNA alkylation in cancer cells.
Authors: Zou, Yang and Huang, Daipeng and He, Shan and Song, Xuefang and Liu, Weijian and Sun, Wen and Du, Jianjun and Fan, Jiangli and Peng, Xiaojun
Journal: Chemical science (2023): 1010-1017
Hydroxyapatite nanoparticles promote mitochondrial-based pyroptosis via activating calcium homeostasis and redox imbalance in vascular smooth muscle cells.
Authors: Xia, Yubin and Li, Bohou and Zhang, Fengxia and Wu, Qiong and Wen, Sichun and Jiang, Nan and Liu, Ding and Huang, Cong and Liu, Shuangxin
Journal: Nanotechnology (2022)
Accurate identification of kidney injury progression via a fluorescent biosensor array.
Authors: Yu, Xie-An and Zhang, Lei and Zhang, Ran and Bai, Xuefei and Zhang, Ying and Hu, Yiting and Wu, Yang and Li, Ziyi and Wang, Bing and Tian, Jiangwei
Journal: Mikrochimica acta (2022): 304
Near-Infrared Dual-Emission Ratiometric Fluorescence Imaging Nanoprobe for Real-Time Tracing the Generation of Endogenous Peroxynitrite in Single Living Cells and In Vivo.
Authors: Lin, Pengxiang and Chen, Dongxia and Zhang, Liangliang and Xu, Jiayao and Huang, Yong and Zhao, Shulin
Journal: ACS omega (2020): 13278-13286
Single-Molecule FRET Detection of Sub-Nanometer Distance Changes in the Range below a 3-Nanometer Scale.
Authors: Son, Heyjin and Mo, Woori and Park, Jaeil and Lee, Joong-Wook and Lee, Sanghwa
Journal: Biosensors (2020)
Page updated on November 3, 2024

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Catalog Number2116
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Physical properties

Solvent

MeCN

Spectral properties

Correction Factor (260 nm)

0.229

Correction Factor (280 nm)

0.193

Extinction coefficient (cm -1 M -1)

75,0001

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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