Tide Quencher™ 7.1WS alkyne [TQ7.1WS alkyne]
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1015.10 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 7.1WS amine [TQ7.1WS amine] |
Tide Quencher™ 7.1WS maleimide [TQ7.1WS maleimide] |
Tide Quencher™ 7.1WS acid [TQ7.1WS acid] |
Tide Quencher™ 7.1WS azide [TQ7.1WS azide] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1015.10 |
Tide Quencher™ 7.1WS (TQ7.1WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.1WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.1WS Alkyne is an excellent building block for preparing TQ7.1WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.1WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.1WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.1WS alkyne [TQ7.1WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.512 µL | 492.562 µL | 985.125 µL | 4.926 mL | 9.851 mL |
5 mM | 19.702 µL | 98.512 µL | 197.025 µL | 985.125 µL | 1.97 mL |
10 mM | 9.851 µL | 49.256 µL | 98.512 µL | 492.562 µL | 985.125 µL |
Molarity calculator
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Images
References
View all 31 references: Citation Explorer
pH-responsive graphene oxide loaded with targeted peptide and anticancer drug for OSCC therapy.
Authors: Li, Ran and Gao, Ruifang and Zhao, Yingjiao and Zhang, Fang and Wang, Xiangyu and Li, Bing and Wang, Lu and Ma, Lixin and Du, Jie
Journal: Frontiers in oncology (2022): 930920
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Journal: Frontiers in oncology (2022): 930920
Near-Infrared Fluorescence Imaging of Carotid Plaques in an Atherosclerotic Murine Model.
Authors: Wu, Xiaotian and Daniel Ulumben, Amy and Long, Steven and Katagiri, Wataru and Wilks, Moses Q and Yuan, Hushan and Cortese, Brian and Yang, Chengeng and Kashiwagi, Satoshi and Choi, Hak Soo and Normandin, Marc D and El Fakhri, Georges and Zaman, Raiyan T
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Journal: Biomolecules (2021)
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Journal: Analytical chemistry (2020)
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CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.
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Journal: EBioMedicine (2020): 102783
Mechanistic profiling of the release kinetics of siRNA from lipidoid-polymer hybrid nanoparticles in vitro and in vivo after pulmonary administration.
Authors: Thanki, Kaushik and van Eetvelde, Delphine and Geyer, Antonia and Fraire, Juan and Hendrix, Remi and Van Eygen, Hannelore and Putteman, Emma and Sami, Haider and de Souza Carvalho-Wodarz, Cristiane and Franzyk, Henrik and Nielsen, Hanne Mørck and Braeckmans, Kevin and Lehr, Claus-Michael and Ogris, Manfred and Foged, Camilla
Journal: Journal of controlled release : official journal of the Controlled Release Society (2019): 82-93
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Journal: Journal of controlled release : official journal of the Controlled Release Society (2019): 82-93
Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging.
Authors: Duranti, Claudia and Carraresi, Laura and Sette, Angelica and Stefanini, Matteo and Lottini, Tiziano and Crescioli, Silvia and Crociani, Olivia and Iamele, Luisa and De Jonge, Hugo and Gherardi, Ermanno and Arcangeli, Annarosa
Journal: Oncotarget (2018): 34972-34989
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Journal: Oncotarget (2018): 34972-34989
Enhanced Release of Molecules upon Ultraviolet (UV) Light Irradiation from Photoresponsive Hydrogels Prepared from Bifunctional Azobenzene and Four-Arm Poly(ethylene glycol).
Authors: Rastogi, Shiva K and Anderson, Hailee E and Lamas, Joseph and Barret, Scott and Cantu, Travis and Zauscher, Stefan and Brittain, William J and Betancourt, Tania
Journal: ACS applied materials & interfaces (2018): 30071-30080
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Journal: ACS applied materials & interfaces (2018): 30071-30080
Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 when Used for Hepatocellular Carcinoma Cell Imaging.
Authors: Dong, Lili and Zhou, Hongxin and Zhao, Menglong and Gao, Xinghui and Liu, Yang and Liu, Dongli and Guo, Wei and Hu, Hongwei and Xie, Qian and Fan, Jia and Lin, Jiang and Wu, Weizhong
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Journal: Molecular therapy. Nucleic acids (2018): 376-386
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Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239