Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne]
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1015.10 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 7.2WS amine [TQ7.2WS amine] |
Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide] |
Tide Quencher™ 7.2WS acid [TQ7.2WS acid] |
Tide Quencher™ 7.2WS azide [TQ7.2WS azide] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1015.10 |
Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS Alkyne is an excellent building block for preparing TQ7.2WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.512 µL | 492.562 µL | 985.125 µL | 4.926 mL | 9.851 mL |
5 mM | 19.702 µL | 98.512 µL | 197.025 µL | 985.125 µL | 1.97 mL |
10 mM | 9.851 µL | 49.256 µL | 98.512 µL | 492.562 µL | 985.125 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 19 references: Citation Explorer
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
The Dual-Targeted Peptide Conjugated Probe for Depicting Residual Nasopharyngeal Carcinoma and Guiding Surgery.
Authors: Huang, Wenhui and He, Zicong and Cai, Xuekang and Zhang, Jingming and Li, Wei and Wang, Kun and Zhang, Shuixing
Journal: Biosensors (2022)
Authors: Huang, Wenhui and He, Zicong and Cai, Xuekang and Zhang, Jingming and Li, Wei and Wang, Kun and Zhang, Shuixing
Journal: Biosensors (2022)
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Measuring Kidney Perfusion, pH, and Renal Clearance Consecutively Using MRI and Multispectral Optoacoustic Tomography.
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Evaluation of Targeting Efficiency of Joints with Anticollagen II Antibodies.
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
Authors: Lofchy, Laren A and Vu, Vivian P and Banda, Nirmal K and Ramirez, Joseline Ramos and Smith, Weston J and Gifford, Geoffrey and Gaikwad, Hanmant and Scheinman, Robert I and Simberg, Dmitri
Journal: Molecular pharmaceutics (2019): 2445-2451
Near-infrared fluorescence laparoscopy of the ureter with three preclinical dyes in a pig model.
Authors: van den Bos, Jacqueline and Al-Taher, Mahdi and Bouvy, Nicole D and Stassen, Laurents P S
Journal: Surgical endoscopy (2019): 986-991
Authors: van den Bos, Jacqueline and Al-Taher, Mahdi and Bouvy, Nicole D and Stassen, Laurents P S
Journal: Surgical endoscopy (2019): 986-991
Evaluation of a novel dye for near-infrared fluorescence delineation of the ureters during laparoscopy.
Authors: Al-Taher, M and van den Bos, J and Schols, R M and Kubat, B and Bouvy, N D and Stassen, L P S
Journal: BJS open (2018): 254-261
Authors: Al-Taher, M and van den Bos, J and Schols, R M and Kubat, B and Bouvy, N D and Stassen, L P S
Journal: BJS open (2018): 254-261
A Modular Dual-Labeling Scaffold That Retains Agonistic Properties for Somatostatin Receptor Targeting.
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Authors: Ghosh, Sukhen C and Rodriguez, Melissa and Carmon, Kendra S and Voss, Julie and Wilganowski, Nathaniel L and Schonbrunn, Agnes and Azhdarinia, Ali
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2017): 1858-1864
Longitudinal monitoring of skin accumulation of nanocarriers and biologicals with fiber optic near infrared fluorescence spectroscopy (FONIRS).
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174
Authors: Griffin, James I and Benchimol, Michael J and Simberg, Dmitri
Journal: Journal of controlled release : official journal of the Controlled Release Society (2017): 167-174