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Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne]

Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS Alkyne is an excellent building block for preparing TQ7.2WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.

References

View all 19 references: Citation Explorer
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
The Dual-Targeted Peptide Conjugated Probe for Depicting Residual Nasopharyngeal Carcinoma and Guiding Surgery.
Authors: Huang, Wenhui and He, Zicong and Cai, Xuekang and Zhang, Jingming and Li, Wei and Wang, Kun and Zhang, Shuixing
Journal: Biosensors (2022)
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Measuring Kidney Perfusion, pH, and Renal Clearance Consecutively Using MRI and Multispectral Optoacoustic Tomography.
Authors: Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish
Journal: Molecular imaging and biology (2020): 494-503
Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.
Authors: Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke
Journal: ACS omega (2020): 13829-13839
Page updated on October 9, 2024

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Catalog Number2129
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Physical properties

Molecular weight

1015.10

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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