Tide Quencher™ 7.2WS amine [TQ7.2WS amine]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1134.14 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide] |
Tide Quencher™ 7.2WS acid [TQ7.2WS acid] |
Tide Quencher™ 7.2WS azide [TQ7.2WS azide] |
Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1134.14 |
Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS amine is primarily used for labeling biomolecules that contain a carbonyl group. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.2WS amine [TQ7.2WS amine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 88.173 µL | 440.863 µL | 881.725 µL | 4.409 mL | 8.817 mL |
5 mM | 17.635 µL | 88.173 µL | 176.345 µL | 881.725 µL | 1.763 mL |
10 mM | 8.817 µL | 44.086 µL | 88.173 µL | 440.863 µL | 881.725 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 16 references: Citation Explorer
FRET causing misleading signal from fluorescein excited by the violet laser in flow cytometry.
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
Authors: Waeckel, Louis and Khenine, Hana and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2023)
Stepwise Energy Transfer: Near-Infrared Persistent Luminescence from Doped Polymeric Systems.
Authors: Lin, Faxu and Wang, Haiyang and Cao, Yifeng and Yu, Rujun and Liang, Guodong and Huang, Huahua and Mu, Yingxiao and Yang, Zhiyong and Chi, Zhenguo
Journal: Advanced materials (Deerfield Beach, Fla.) (2022): e2108333
Authors: Lin, Faxu and Wang, Haiyang and Cao, Yifeng and Yu, Rujun and Liang, Guodong and Huang, Huahua and Mu, Yingxiao and Yang, Zhiyong and Chi, Zhenguo
Journal: Advanced materials (Deerfield Beach, Fla.) (2022): e2108333
Fluorescent energy transfer causing misleading signal in multicolor flow cytometry.
Authors: Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
Authors: Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
PI3KC3 complex subunit NRBF2 is required for apoptotic cell clearance to restrict intestinal inflammation.
Authors: Wu, Ming-Yue and Liu, Le and Wang, Er-Jin and Xiao, Hai-Tao and Cai, Cui-Zan and Wang, Jing and Su, Huanxing and Wang, Yitao and Tan, Jieqiong and Zhang, Zhuohua and Wang, Juan and Yao, Maojing and Ouyang, De-Fang and Yue, Zhenyu and Li, Min and Chen, Ye and Bian, Zhao-Xiang and Lu, Jia-Hong
Journal: Autophagy (2020): 1-16
Authors: Wu, Ming-Yue and Liu, Le and Wang, Er-Jin and Xiao, Hai-Tao and Cai, Cui-Zan and Wang, Jing and Su, Huanxing and Wang, Yitao and Tan, Jieqiong and Zhang, Zhuohua and Wang, Juan and Yao, Maojing and Ouyang, De-Fang and Yue, Zhenyu and Li, Min and Chen, Ye and Bian, Zhao-Xiang and Lu, Jia-Hong
Journal: Autophagy (2020): 1-16
Single-Molecule FRET Detection of Sub-Nanometer Distance Changes in the Range below a 3-Nanometer Scale.
Authors: Son, Heyjin and Mo, Woori and Park, Jaeil and Lee, Joong-Wook and Lee, Sanghwa
Journal: Biosensors (2020)
Authors: Son, Heyjin and Mo, Woori and Park, Jaeil and Lee, Joong-Wook and Lee, Sanghwa
Journal: Biosensors (2020)
[Development of Novel Dark Quenchers and Their Application to Imaging Probes].
Authors: Hanaoka, Kenjiro
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2019): 277-283
Authors: Hanaoka, Kenjiro
Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (2019): 277-283
Competition of Charge and Energy Transfer Processes in Donor-Acceptor Fluorescence Pairs: Calibrating the Spectroscopic Ruler.
Authors: Moroz, Pavel and Jin, Zhicheng and Sugiyama, Yuya and Lara, D'Andree and Razgoniaeva, Natalia and Yang, Mingrui and Kholmicheva, Natalia and Khon, Dmitriy and Mattoussi, Hedi and Zamkov, Mikhail
Journal: ACS nano (2018): 5657-5665
Authors: Moroz, Pavel and Jin, Zhicheng and Sugiyama, Yuya and Lara, D'Andree and Razgoniaeva, Natalia and Yang, Mingrui and Kholmicheva, Natalia and Khon, Dmitriy and Mattoussi, Hedi and Zamkov, Mikhail
Journal: ACS nano (2018): 5657-5665
Bioorthogonally Applicable Fluorescence Deactivation Strategy for Receptor Kinetics Study and Theranostic Pretargeting Approaches.
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
Authors: van der Wal, Steffen and de Korne, Clarize M and Sand, Laurens G L and van Willigen, Danny M and Hogendoorn, Pancras C W and Szuhai, Karoly and van Leeuwen, Fijs W B and Buckle, Tessa
Journal: Chembiochem : a European journal of chemical biology (2018)
FRET Imaging of Enzyme-Responsive HPMA Copolymer Conjugate.
Authors: Zhang, Rui and Yang, Jiyuan and Radford, D Christopher and Fang, Yixin and Kopeček, Jindřich
Journal: Macromolecular bioscience (2017)
Authors: Zhang, Rui and Yang, Jiyuan and Radford, D Christopher and Fang, Yixin and Kopeček, Jindřich
Journal: Macromolecular bioscience (2017)
Development of a series of near-infrared dark quenchers based on Si-rhodamines and their application to fluorescent probes.
Authors: Myochin, Takuya and Hanaoka, Kenjiro and Iwaki, Shimpei and Ueno, Tasuku and Komatsu, Toru and Terai, Takuya and Nagano, Tetsuo and Urano, Yasuteru
Journal: Journal of the American Chemical Society (2015): 4759-65
Authors: Myochin, Takuya and Hanaoka, Kenjiro and Iwaki, Shimpei and Ueno, Tasuku and Komatsu, Toru and Terai, Takuya and Nagano, Tetsuo and Urano, Yasuteru
Journal: Journal of the American Chemical Society (2015): 4759-65