Tide Quencher™ 7.2WS azide [TQ7.2WS azide]
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1046.12 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 7.2WS amine [TQ7.2WS amine] |
Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide] |
Tide Quencher™ 7.2WS acid [TQ7.2WS acid] |
Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1046.12 |
Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS Azide is an excellent building block for preparing TQ5.1WS-labeled probes from alkyne-modified oligonucleotides (including DBCO-modified oligonucleotides) and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7.2WS azide [TQ7.2WS azide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 95.591 µL | 477.957 µL | 955.913 µL | 4.78 mL | 9.559 mL |
5 mM | 19.118 µL | 95.591 µL | 191.183 µL | 955.913 µL | 1.912 mL |
10 mM | 9.559 µL | 47.796 µL | 95.591 µL | 477.957 µL | 955.913 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
References
View all 50 references: Citation Explorer
Paired-agent imaging as a rapid en face margin screening method in Mohs micrographic surgery.
Authors: Torres, Veronica C and Hodge, Sassan and Levy, Joshua J and Vaickus, Louis J and Chen, Eunice Y and LeBouef, Matthew and Samkoe, Kimberley S
Journal: Frontiers in oncology (2023): 1196517
Authors: Torres, Veronica C and Hodge, Sassan and Levy, Joshua J and Vaickus, Louis J and Chen, Eunice Y and LeBouef, Matthew and Samkoe, Kimberley S
Journal: Frontiers in oncology (2023): 1196517
Noninvasive Imaging OX40+ Activated T Cells Provides Early Warning of Rheumatoid Arthritis.
Authors: Wen, Gang and Lei, Hongwei and Qi, Baochang and Duan, Shao and Xiao, Zunyu and Han, Chaozhe and Xia, Yifei and Jing, Chengwei and Liu, Jianyu and Li, Chao
Journal: Molecular imaging and biology (2023): 621-629
Authors: Wen, Gang and Lei, Hongwei and Qi, Baochang and Duan, Shao and Xiao, Zunyu and Han, Chaozhe and Xia, Yifei and Jing, Chengwei and Liu, Jianyu and Li, Chao
Journal: Molecular imaging and biology (2023): 621-629
Quantifying imaging agent binding and dissociation in 3D cancer spheroid tissue culture using paired-agent principles.
Authors: Li, Chengyue and Torres, Veronica C and He, Yusheng and Xu, Xiaochun and Papavasiliou, Georgia and Samkoe, Kimberley S and Brankov, Jovan G and Tichauer, Kenneth M
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Authors: Li, Chengyue and Torres, Veronica C and He, Yusheng and Xu, Xiaochun and Papavasiliou, Georgia and Samkoe, Kimberley S and Brankov, Jovan G and Tichauer, Kenneth M
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Preclinical Identification Of Tumor-Draining Lymph Nodes Using a Multimodal Non-invasive In vivo Imaging Approach.
Authors: Knopf, Philipp and Stowbur, Dimitri and Hoffmann, Sabrina H L and Fransen, Marieke F and Schwenck, Johannes and Pichler, Bernd J and Kneilling, Manfred
Journal: Molecular imaging and biology (2023): 606-618
Authors: Knopf, Philipp and Stowbur, Dimitri and Hoffmann, Sabrina H L and Fransen, Marieke F and Schwenck, Johannes and Pichler, Bernd J and Kneilling, Manfred
Journal: Molecular imaging and biology (2023): 606-618
Imaging the Alternatively Spliced D Domain of Tenascin C in a Preclinical Model of Inflammatory Bowel Disease.
Authors: Zhang, Liang and Wang, Yuzhen and Homan, Kristoff T and Gaudette, Stephanie M and McCluskey, Andrew J and Chan, Ying and Murphy, Joanne and Abdalla, Mary and Nelson, Christine M and Sun, Victor Z and Erickson, Jamie E and Knight, Heather L and Clabbers, Anca and Sterman, Annette J Schwartz and Mitra, Soumya
Journal: Molecular imaging and biology (2023): 314-323
Authors: Zhang, Liang and Wang, Yuzhen and Homan, Kristoff T and Gaudette, Stephanie M and McCluskey, Andrew J and Chan, Ying and Murphy, Joanne and Abdalla, Mary and Nelson, Christine M and Sun, Victor Z and Erickson, Jamie E and Knight, Heather L and Clabbers, Anca and Sterman, Annette J Schwartz and Mitra, Soumya
Journal: Molecular imaging and biology (2023): 314-323
Identification of a Suitable Untargeted Agent for the Clinical Translation of ABY-029 Paired-Agent Imaging in Fluorescence-Guided Surgery.
Authors: Wang, Cheng and Xu, Xiaochun and Hodge, Sassan and Chen, Eunice Y and Hoopes, P Jack and Tichauer, Kenneth M and Samkoe, Kimberley S
Journal: Molecular imaging and biology (2023): 97-109
Authors: Wang, Cheng and Xu, Xiaochun and Hodge, Sassan and Chen, Eunice Y and Hoopes, P Jack and Tichauer, Kenneth M and Samkoe, Kimberley S
Journal: Molecular imaging and biology (2023): 97-109
Rapid and Quantitative Intraoperative Pathology-Assisted Surgery by Paired-Agent Imaging-Derived Confidence Map.
Authors: Wang, Cheng and Hodge, Sassan and Ravi, Divya and Chen, Eunice Y and Hoopes, P Jack and Tichauer, Kenneth M and Samkoe, Kimberley S
Journal: Molecular imaging and biology (2023): 190-202
Authors: Wang, Cheng and Hodge, Sassan and Ravi, Divya and Chen, Eunice Y and Hoopes, P Jack and Tichauer, Kenneth M and Samkoe, Kimberley S
Journal: Molecular imaging and biology (2023): 190-202
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker.
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Tumor-Specific Imaging with Angiostamp800 or Bevacizumab-IRDye 800CW Improves Fluorescence-Guided Surgery over Indocyanine Green in Peritoneal Carcinomatosis.
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Fluorophore-conjugated 4-1BB antibody enables early detection of T-cell responses in inflammatory arthritis via NIRF imaging.
Authors: Duan, Shao and Han, Chaozhe and Xia, Yifei and Jing, Chengwei and Dong, Bohan and Zhang, Xin and Wang, WeiWei and Wang, Yu and Zhang, Maomao and Li, Ping and Chen, Weiyu and Xiao, Zunyu and Li, Chao
Journal: European journal of nuclear medicine and molecular imaging (2022): 38-47
Authors: Duan, Shao and Han, Chaozhe and Xia, Yifei and Jing, Chengwei and Dong, Bohan and Zhang, Xin and Wang, WeiWei and Wang, Yu and Zhang, Maomao and Li, Ping and Chen, Weiyu and Xiao, Zunyu and Li, Chao
Journal: European journal of nuclear medicine and molecular imaging (2022): 38-47