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Tide Quencher™ 7.2WS azide [TQ7.2WS azide]

Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS Azide is an excellent building block for preparing TQ5.1WS-labeled probes from alkyne-modified oligonucleotides (including DBCO-modified oligonucleotides) and peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.

References

View all 50 references: Citation Explorer
Paired-agent imaging as a rapid en face margin screening method in Mohs micrographic surgery.
Authors: Torres, Veronica C and Hodge, Sassan and Levy, Joshua J and Vaickus, Louis J and Chen, Eunice Y and LeBouef, Matthew and Samkoe, Kimberley S
Journal: Frontiers in oncology (2023): 1196517
Noninvasive Imaging OX40+ Activated T Cells Provides Early Warning of Rheumatoid Arthritis.
Authors: Wen, Gang and Lei, Hongwei and Qi, Baochang and Duan, Shao and Xiao, Zunyu and Han, Chaozhe and Xia, Yifei and Jing, Chengwei and Liu, Jianyu and Li, Chao
Journal: Molecular imaging and biology (2023): 621-629
Quantifying imaging agent binding and dissociation in 3D cancer spheroid tissue culture using paired-agent principles.
Authors: Li, Chengyue and Torres, Veronica C and He, Yusheng and Xu, Xiaochun and Papavasiliou, Georgia and Samkoe, Kimberley S and Brankov, Jovan G and Tichauer, Kenneth M
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Preclinical Identification Of Tumor-Draining Lymph Nodes Using a Multimodal Non-invasive In vivo Imaging Approach.
Authors: Knopf, Philipp and Stowbur, Dimitri and Hoffmann, Sabrina H L and Fransen, Marieke F and Schwenck, Johannes and Pichler, Bernd J and Kneilling, Manfred
Journal: Molecular imaging and biology (2023): 606-618
Imaging the Alternatively Spliced D Domain of Tenascin C in a Preclinical Model of Inflammatory Bowel Disease.
Authors: Zhang, Liang and Wang, Yuzhen and Homan, Kristoff T and Gaudette, Stephanie M and McCluskey, Andrew J and Chan, Ying and Murphy, Joanne and Abdalla, Mary and Nelson, Christine M and Sun, Victor Z and Erickson, Jamie E and Knight, Heather L and Clabbers, Anca and Sterman, Annette J Schwartz and Mitra, Soumya
Journal: Molecular imaging and biology (2023): 314-323
Page updated on November 9, 2024

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Catalog Number2128
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Physical properties

Molecular weight

1046.12

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure