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Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide]

Tide Quencher™ 7.2WS (TQ7.2WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of common NIR fluorophores such as Cy7, Alexa Fluor® 700, Alexa Fluor® 750, iFluor® 700, iFluor® 710, iFluor® 720 and iFluor® 750. It is an improved version of TQ7 and BHQ3. TQ7.2WS is designed to be a superior quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for NIR dyes. Tide Quencher™ 7.2WS maleimide is primarily used for the post-labeling of thiol-modified oligonucleotides and the Cys residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ7.2WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ7.2WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.

References

View all 2 references: Citation Explorer
Visualising long distance sugar transport in fungi using infrared fluorescence scanning imaging.
Authors: Bleichrodt, Robert-Jan and Wösten, Han A B
Journal: Fungal genetics and biology : FG & B (2022): 103699
Electrophoretic mobility shift scanning using an automated infrared DNA sequencer.
Authors: Sano, M and Ohyama, A and Takase, K and Yamamoto, M and Machida, M
Journal: BioTechniques (2001): 1056-8, 1060, 1062
Page updated on December 11, 2024

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Catalog Number2126
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Physical properties

Molecular weight

1100.16

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure