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Tide Quencher™ 7WS maleimide [TQ7WS maleimide]

Dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
Dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
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Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weight1109.24
SolventDMSO
Spectral properties
Absorbance (nm)764
Correction Factor (260 nm)0.072
Correction Factor (280 nm)0.091
Extinction coefficient (cm -1 M -1)140000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Tide Quencher™ 2WS acid [TQ2WS acid]
Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE]
Tide Quencher™ 4WS acid [TQ4WS acid]
Tide Quencher™ 4WS amine [TQ4WS amine]
Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å*
Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å*
Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE]
Tide Quencher™ 4WS azide [TQ4WS azide]
Tide Quencher™ 4WS alkyne [TQ4WS alkyne]
Tide Quencher™ 5WS acid [TQ5WS acid]
Tide Quencher™ 5WS amine [TQ5WS amine]
Tide Quencher™ 5 CPG [TQ5 CPG] *500 Å*
Tide Quencher™ 5 CPG [TQ5 CPG] *1000 Å*
Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE]
Tide Quencher™ 5WS alkyne [TQ5WS alkyne]
Tide Quencher™ 6WS acid [TQ6WS acid]
Tide Quencher™ 6WS amine [TQ6WS amine]
Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE]
Tide Quencher™ 6WS azide [TQ6WS azide]
Tide Quencher™ 6WS alkyne [TQ6WS alkyne]
Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE]
Tide Quencher™ 1 azide [TQ1 azide]
Tide Quencher™ 1 alkyne [TQ1 alkyne]
Tide Quencher™ 1 acid [TQ1 acid]
Tide Quencher™ 1 amine [TQ1 amine]
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å*
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å*
Tide Quencher™ 1 phosphoramidite [TQ1 phosphoramidite]
Tide Quencher™ 1 succinimidyl ester [TQ1 SE]
Tide Quencher™ 2 acid [TQ2 acid]
Tide Quencher™ 2 amine [TQ2 amine]
Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å*
Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å*
Tide Quencher™ 2 phosphoramidite [TQ2 phosphoramidite]
Tide Quencher™ 2 succinimidyl ester [TQ2 SE]
Tide Quencher™ 2 azide [TQ2 azide]
Tide Quencher™ 2 alkyne [TQ2 alkyne]
Tide Quencher™ 3 acid [TQ3 acid]
Tide Quencher™ 3 amine [TQ3 amine]
Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å*
Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å*
Tide Quencher™ 3WS acid [TQ3WS acid]
Tide Quencher™ 3 phosphoramidite [TQ3 phosphoramidite]
Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE]
Tide Quencher™ 3 succinimidyl ester [TQ3 SE]
Tide Quencher™ 3 azide [TQ3 azide]
Tide Quencher™ 3 alkyne [TQ3 alkyne]
Tide Quencher™ 2WS alkyne [TQ2WS alkyne]
Tide Quencher™ 4WS-DBCO [TQ4WS-DBCO]
Tide Quencher™ 5WS azide [TQ5WS azide]
Tide Quencher™ 5.1WS acid [TQ5.1WS acid]
Tide Quencher™ 5.1WS amine [TQ5.1WS amine]
Tide Quencher™ 5.1 CPG [TQ5.1 CPG] *500 Å*
Tide Quencher™ 5.1 CPG [TQ5.1 CPG] *1000 Å*
Tide Quencher™ 5.1WS maleimide [TQ5.1WS maleimide]
Tide Quencher™ 5.1WS succinimidyl ester [TQ5.1WS SE]
Tide Quencher™ 5.1WS azide [TQ5.1WS azide]
Tide Quencher™ 5.1WS alkyne [TQ5.1WS alkyne]
Tide Quencher™ 7.1WS acid [TQ7.1WS acid]
Tide Quencher™ 7.1WS amine [TQ7.1WS amine]
Tide Quencher™ 7.1 CPG [TQ7.1 CPG] *500 Å*
Tide Quencher™ 7.1 CPG [TQ7.1 CPG] *1000 Å*
Tide Quencher™ 7.1WS maleimide [TQ7.1WS maleimide]
Tide Quencher™ 7.1WS succinimidyl ester [TQ7.1WS SE]
Tide Quencher™ 7.1WS azide [TQ7.1WS azide]
Tide Quencher™ 7.1WS alkyne [TQ7.1WS alkyne]
Tide Quencher™ 7.2WS acid [TQ7.2WS acid]
Tide Quencher™ 7.2WS amine [TQ7.2WS amine]
Tide Quencher™ 7.2 CPG [TQ7.2 CPG] *500 Å*
Tide Quencher™ 7.2 CPG [TQ7.2 CPG] *1000 Å*
Tide Quencher™ 7.2WS maleimide [TQ7.2WS maleimide]
Tide Quencher™ 7.2WS succinimidyl ester [TQ7.2WS SE]
Tide Quencher™ 7.2WS azide [TQ7.2WS azide]
Tide Quencher™ 7.2WS alkyne [TQ7.2WS alkyne]
Tide Quencher™ 8WS acid [TQ8WS acid]
Tide Quencher™ 8WS amine [TQ8WS amine]
Tide Quencher™ 8 CPG [TQ8 CPG] *500 Å*
Tide Quencher™ 8WS succinimidyl ester [TQ8WS SE]
Tide Quencher™ 8WS azide [TQ8WS azide]
Tide Quencher™ 8WS alkyne [TQ8WS alkyne]
Tide Quencher™ 8 CPG [TQ8 CPG] *1000 Å*
Show More (71)

OverviewpdfSDSpdfProtocol


Molecular weight
1109.24
Absorbance (nm)
764
Correction Factor (260 nm)
0.072
Correction Factor (280 nm)
0.091
Extinction coefficient (cm -1 M -1)
140000
TQ7WS is designed to be a superior quencher to Cy7, TF7 and Alexa Fluor 750. TQ7WS has (a). much stronger absorption; (b). much higher quenching efficiency; and (c). versatile reactive forms with desired solubility for labeling oligonucleotides and peptides. This TQWS product is used for post-labeling of thiol-modified oligonucleotides and cysteine-containing peptides.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 7WS maleimide [TQ7WS maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM90.152 µL450.759 µL901.518 µL4.508 mL9.015 mL
5 mM18.03 µL90.152 µL180.304 µL901.518 µL1.803 mL
10 mM9.015 µL45.076 µL90.152 µL450.759 µL901.518 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)764
Correction Factor (260 nm)0.072
Correction Factor (280 nm)0.091
Extinction coefficient (cm -1 M -1)140000

Images


Citations


View all 7 citations: Citation Explorer
A mechanistic model to predict effects of cathepsin B and cystatin C on &beta;-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc--M117
Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310
Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82--e1
Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: ECS Transactions (2015): 9--16
Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264--9272
Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: ChemBioChem (2011): 2217--2226
FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)
Authors: Soriani, Frederico Marianetti and Russo, Remo Castro

References


View all 25 references: Citation Explorer
Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Photochem Photobiol Sci (2009): 1202
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: Bioconjug Chem (2009): 147
The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer/quantum dot bioconjugates
Authors: Kim GI, Kim KW, Oh MK, Sung YM.
Journal: Nanotechnology (2009): 175503
Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702
Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: Andreoni A, Bondani M, Nardo L.
Journal: Mol Cell Probes (2009): 119
Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: J Med Chem (2009): 358
Rapid detection and quantification of Propionibacteriaceae
Authors: Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: Br J Ophthalmol (2009): 258
Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519