Tide Quencher™ 8 CPG [TQ8 CPG] *500 Å*
Tide Quencher™ 8 (TQ8) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8 is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8 CPG is an excellent building block for preparing TQ8-labeled oligonucleotides. The oligo prepared from (TQ8 CPG) may be deprotected in 0.05M potassium carbonate in methanol for 4 hours at room temperature for 2 hours. Alternatively, the oligo may be deprotected in ammonium hydroxide at room temperature for 24-36 hours. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8 quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified.
Product family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4 CPG [TQ4 CPG] *500 Å* | 23000 | 0.146 | 0.183 |
Tide Quencher™ 4 CPG [TQ4 CPG] *1000 Å* | 23000 | 0.146 | 0.183 |
Tide Quencher™ 1 CPG [TQ1 CPG] *500 Å* | 20000 | 0.147 | 0.194 |
Tide Quencher™ 1 CPG [TQ1 CPG] *1000 Å* | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 CPG [TQ2 CPG] *500 Å* | 21000 | 0.100 | 0.12 |
Tide Quencher™ 2 CPG [TQ2 CPG] *1000 Å* | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 CPG [TQ3 CPG] *500 Å* | 22000 | 0.085 | 0.091 |
Tide Quencher™ 3 CPG [TQ3 CPG] *1000 Å* | 22000 | 0.085 | 0.091 |
References
View all 50 references: Citation Explorer
Specific Targeting and Labeling of Colonic Polyps in CPC-APC Mice with Mucin 5AC Fluorescent Antibodies: A Model for Detection of Early Colon Cancer.
Authors: Turner, Michael A and Cox, Kristin E and Liu, Shanglei and Neel, Nicholas and Amirfakhri, Siamak and Nishino, Hiroto and Hosseini, Mojgan and Alcantara, Joshua A and Abd El-Hafeez, Amer Ali and Lwin, Thinzar M and Mallya, Kavita and Pisegna, Joseph R and Singh, Satish K and Ghosh, Pradipta and Hoffman, Robert M and Batra, Surinder K and Bouvet, Michael
Journal: Current issues in molecular biology (2023): 3347-3358
Authors: Turner, Michael A and Cox, Kristin E and Liu, Shanglei and Neel, Nicholas and Amirfakhri, Siamak and Nishino, Hiroto and Hosseini, Mojgan and Alcantara, Joshua A and Abd El-Hafeez, Amer Ali and Lwin, Thinzar M and Mallya, Kavita and Pisegna, Joseph R and Singh, Satish K and Ghosh, Pradipta and Hoffman, Robert M and Batra, Surinder K and Bouvet, Michael
Journal: Current issues in molecular biology (2023): 3347-3358
Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study.
Authors: Chiti, Lavinia E and Husi, Benjamin and Park, Brian and Beer, Patricia and D'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Frontiers in veterinary science (2023): 1091842
Authors: Chiti, Lavinia E and Husi, Benjamin and Park, Brian and Beer, Patricia and D'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Frontiers in veterinary science (2023): 1091842
Optimizing Axial and Peripheral Substitutions in Si-Centered Naphthalocyanine Dyes for Enhancing Aqueous Solubility and Photoacoustic Signal Intensity.
Authors: Saad, Mohammad Ahsan and Pawle, Robert and Selfridge, Scott and Contreras, Leslie and Xavierselvan, Marvin and Nguyen, Christopher D and Mallidi, Srivalleesha and Hasan, Tayyaba
Journal: International journal of molecular sciences (2023)
Authors: Saad, Mohammad Ahsan and Pawle, Robert and Selfridge, Scott and Contreras, Leslie and Xavierselvan, Marvin and Nguyen, Christopher D and Mallidi, Srivalleesha and Hasan, Tayyaba
Journal: International journal of molecular sciences (2023)
Preclinical assessment of IRDye800CW-labeled gastrin-releasing peptide receptor-targeting peptide for near infrared-II imaging of brain malignancies.
Authors: Zhang, Yuan and Wang, Li and Zhang, Chengkai and Zhang, Jingjing and Yuan, Linhao and Jin, Shucheng and Zhou, Wenjianlong and Guan, Xiudong and Kang, Peng and Zhang, Chuanbao and Tian, Jie and Chen, Xiaoyuan and Li, Deling and Jia, Wang
Journal: Bioengineering & translational medicine (2023): e10532
Authors: Zhang, Yuan and Wang, Li and Zhang, Chengkai and Zhang, Jingjing and Yuan, Linhao and Jin, Shucheng and Zhou, Wenjianlong and Guan, Xiudong and Kang, Peng and Zhang, Chuanbao and Tian, Jie and Chen, Xiaoyuan and Li, Deling and Jia, Wang
Journal: Bioengineering & translational medicine (2023): e10532
Enhancing intraoperative tumor delineation with multispectral short-wave infrared fluorescence imaging and machine learning.
Authors: Waterhouse, Dale J and Privitera, Laura and Anderson, John and Stoyanov, Danail and Giuliani, Stefano
Journal: Journal of biomedical optics (2023): 094804
Authors: Waterhouse, Dale J and Privitera, Laura and Anderson, John and Stoyanov, Danail and Giuliani, Stefano
Journal: Journal of biomedical optics (2023): 094804
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