Tide Quencher™ 8WS alkyne [TQ8WS alkyne]
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS Alkyne is an excellent building block for preparing TQ8WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Product family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS alkyne [TQ4WS alkyne] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS alkyne [TQ5WS alkyne] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS alkyne [TQ6WS alkyne] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS alkyne [TQ7WS alkyne] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 alkyne [TQ1 alkyne] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 alkyne [TQ2 alkyne] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 alkyne [TQ3 alkyne] | 22000 | 0.085 | 0.091 |
Tide Quencher™ 2WS alkyne [TQ2WS alkyne] | 48000 | 0.100 | 0.120 |
Tide Fluor™ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission* | 250000 | - | 0.109 |
References
View all 50 references: Citation Explorer
Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation.
Authors: Lai, Chenting and Calvarese, Matteo and Reichwald, Karl and Bae, Hyeonsoo and Vafaeinezhad, Mohammadsadegh and Meyer-Zedler, Tobias and Hoffmann, Franziska and Mühlig, Anna and Eidam, Tino and Stutzki, Fabian and Messerschmidt, Bernhard and Gross, Herbert and Schmitt, Michael and Guntinas-Lichius, Orlando and Popp, Jürgen
Journal: Journal of biomedical optics (2023): 066004
Authors: Lai, Chenting and Calvarese, Matteo and Reichwald, Karl and Bae, Hyeonsoo and Vafaeinezhad, Mohammadsadegh and Meyer-Zedler, Tobias and Hoffmann, Franziska and Mühlig, Anna and Eidam, Tino and Stutzki, Fabian and Messerschmidt, Bernhard and Gross, Herbert and Schmitt, Michael and Guntinas-Lichius, Orlando and Popp, Jürgen
Journal: Journal of biomedical optics (2023): 066004
Indocyanine Green Imaging in Laparoscopic Cholecystectomy Plus Laparoscopic Common Bile Duct Exploration: A Suitable Option for Patients With Difficult Exploration (With Videos).
Authors: Ma, Chenhui and Zhang, Lei and Wen, Junye and Zhang, Wanxing and Chen, Hao
Journal: Surgical laparoscopy, endoscopy & percutaneous techniques (2023): 235-240
Authors: Ma, Chenhui and Zhang, Lei and Wen, Junye and Zhang, Wanxing and Chen, Hao
Journal: Surgical laparoscopy, endoscopy & percutaneous techniques (2023): 235-240
Current and Future Applications of Fluorescence Guidance in Orthopaedic Surgery.
Authors: Streeter, Samuel S and Hebert, Kendra A and Bateman, Logan M and Ray, Gabrielle S and Dean, Ryan E and Geffken, Kurt T and Resnick, Corey T and Austin, Daniel C and Bell, John-Erik and Sparks, Michael B and Gibbs, Summer L and Samkoe, Kimberley S and Gitajn, I Leah and Elliott, Jonathan Thomas and Henderson, Eric R
Journal: Molecular imaging and biology (2023): 46-57
Authors: Streeter, Samuel S and Hebert, Kendra A and Bateman, Logan M and Ray, Gabrielle S and Dean, Ryan E and Geffken, Kurt T and Resnick, Corey T and Austin, Daniel C and Bell, John-Erik and Sparks, Michael B and Gibbs, Summer L and Samkoe, Kimberley S and Gitajn, I Leah and Elliott, Jonathan Thomas and Henderson, Eric R
Journal: Molecular imaging and biology (2023): 46-57
A novel technique proposition for determining the resection margins in lung resection by using a thermal camera.
Authors: Sayan, Muhammet and Kankoc, Aykut and Valiyev, Elgun and Celik, Ali
Journal: General thoracic and cardiovascular surgery (2023)
Authors: Sayan, Muhammet and Kankoc, Aykut and Valiyev, Elgun and Celik, Ali
Journal: General thoracic and cardiovascular surgery (2023)
Importance of intraoperative indocyanine green imaging in the management of non-occlusive mesenteric ischemia: a case report.
Authors: Miyashita, Ryohei and Kitazawa, Masato and Tokumaru, Shigeo and Nakamura, Satoshi and Koyama, Makoto and Yamamoto, Yuta and Hondo, Nao and Miyazaki, Satoru and Soejima, Yuji
Journal: Surgical case reports (2023): 31
Authors: Miyashita, Ryohei and Kitazawa, Masato and Tokumaru, Shigeo and Nakamura, Satoshi and Koyama, Makoto and Yamamoto, Yuta and Hondo, Nao and Miyazaki, Satoru and Soejima, Yuji
Journal: Surgical case reports (2023): 31
Page updated on October 11, 2024