Tide Quencher™ 8WS alkyne [TQ8WS alkyne]
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 914.97 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 8WS amine [TQ8WS amine] |
Tide Quencher™ 8WS maleimide [TQ8WS maleimide] |
Tide Quencher™ 8WS acid [TQ8WS acid] |
Tide Quencher™ 8WS azide [TQ8WS azide] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 914.97 |
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS Alkyne is an excellent building block for preparing TQ8WS-labeled probes from azido-modified oligonucleotides (including picolyl azide-modified oligonucleotides via the well-known click chemistry. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 8WS alkyne [TQ8WS alkyne] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 109.293 µL | 546.466 µL | 1.093 mL | 5.465 mL | 10.929 mL |
5 mM | 21.859 µL | 109.293 µL | 218.586 µL | 1.093 mL | 2.186 mL |
10 mM | 10.929 µL | 54.647 µL | 109.293 µL | 546.466 µL | 1.093 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS alkyne [TQ4WS alkyne] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS alkyne [TQ5WS alkyne] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS alkyne [TQ6WS alkyne] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS alkyne [TQ7WS alkyne] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 alkyne [TQ1 alkyne] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 alkyne [TQ2 alkyne] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 alkyne [TQ3 alkyne] | 22000 | 0.085 | 0.091 |
Tide Quencher™ 2WS alkyne [TQ2WS alkyne] | 48000 | 0.100 | 0.120 |
Tide Fluor™ 8WS alkyne [TF8WS alkyne] *Near Infrared Emission* | 250000 | - | 0.109 |
Images
References
View all 50 references: Citation Explorer
Design and test of a rigid endomicroscopic system for multimodal imaging and femtosecond laser ablation.
Authors: Lai, Chenting and Calvarese, Matteo and Reichwald, Karl and Bae, Hyeonsoo and Vafaeinezhad, Mohammadsadegh and Meyer-Zedler, Tobias and Hoffmann, Franziska and Mühlig, Anna and Eidam, Tino and Stutzki, Fabian and Messerschmidt, Bernhard and Gross, Herbert and Schmitt, Michael and Guntinas-Lichius, Orlando and Popp, Jürgen
Journal: Journal of biomedical optics (2023): 066004
Authors: Lai, Chenting and Calvarese, Matteo and Reichwald, Karl and Bae, Hyeonsoo and Vafaeinezhad, Mohammadsadegh and Meyer-Zedler, Tobias and Hoffmann, Franziska and Mühlig, Anna and Eidam, Tino and Stutzki, Fabian and Messerschmidt, Bernhard and Gross, Herbert and Schmitt, Michael and Guntinas-Lichius, Orlando and Popp, Jürgen
Journal: Journal of biomedical optics (2023): 066004
Real-time quantification of intestinal perfusion and arterial versus venous occlusion using laser speckle contrast imaging in porcine model.
Authors: Liu, Yao Z and Mehrotra, Saloni and Nwaiwu, Chibueze A and Buharin, Vasiliy E and Oberlin, John and Stolyarov, Roman and Schwaitzberg, Steven D and Kim, Peter C W
Journal: Langenbeck's archives of surgery (2023): 114
Authors: Liu, Yao Z and Mehrotra, Saloni and Nwaiwu, Chibueze A and Buharin, Vasiliy E and Oberlin, John and Stolyarov, Roman and Schwaitzberg, Steven D and Kim, Peter C W
Journal: Langenbeck's archives of surgery (2023): 114
Early identification of life-threatening soft-tissue infection using dynamic fluorescence imaging: first-in-kind clinical study of first-pass kinetics.
Authors: Streeter, Samuel S and Ray, Gabrielle S and Bateman, Logan M and Hebert, Kendra A and Bushee, Fallon E and Rodi, Scott W and Gitajn, I Leah and Ahn, Jaimo and Singhal, Sunil and Martin, Niels D and Bernthal, Nicholas M and Lee, Christopher and Obremskey, William T and Schoenecker, Jonathan G and Elliott, Jonathan Thomas and Henderson, Eric R
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Authors: Streeter, Samuel S and Ray, Gabrielle S and Bateman, Logan M and Hebert, Kendra A and Bushee, Fallon E and Rodi, Scott W and Gitajn, I Leah and Ahn, Jaimo and Singhal, Sunil and Martin, Niels D and Bernthal, Nicholas M and Lee, Christopher and Obremskey, William T and Schoenecker, Jonathan G and Elliott, Jonathan Thomas and Henderson, Eric R
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Utility and usability of laser speckle contrast imaging (LSCI) for displaying real-time tissue perfusion/blood flow in robot-assisted surgery (RAS): comparison to indocyanine green (ICG) and use in laparoscopic surgery.
Authors: Liu, Yao Z and Shah, Shinil K and Sanders, Christina M and Nwaiwu, Chibueze A and Dechert, Alyson F and Mehrotra, Saloni and Schwaitzberg, Steven D and Kim, Peter C W and Wilson, Erik B
Journal: Surgical endoscopy (2023): 4803-4811
Authors: Liu, Yao Z and Shah, Shinil K and Sanders, Christina M and Nwaiwu, Chibueze A and Dechert, Alyson F and Mehrotra, Saloni and Schwaitzberg, Steven D and Kim, Peter C W and Wilson, Erik B
Journal: Surgical endoscopy (2023): 4803-4811
Near-Infrared Imaging of Indocyanine Green Identifies Novel Routes of Lymphatic Drainage from Metacarpophalangeal Joints in Healthy Human Hands.
Authors: Kenney, H Mark and Dieudonne, Gregory and Yee, Seonghwan and Maki, Jeffrey H and Wood, Ronald W and Schwarz, Edward M and Ritchlin, Christopher T and Rahimi, Homaira
Journal: Lymphatic research and biology (2023): 388-395
Authors: Kenney, H Mark and Dieudonne, Gregory and Yee, Seonghwan and Maki, Jeffrey H and Wood, Ronald W and Schwarz, Edward M and Ritchlin, Christopher T and Rahimi, Homaira
Journal: Lymphatic research and biology (2023): 388-395
Fluorescence-guided and molecularly-guided debridement: identifying devitalized and infected tissue in orthopaedic trauma.
Authors: Elliott, Jonathan Thomas and Henderson, Eric and Streeter, Samuel S and Demidov, Valentin and Han, Xinyue and Tang, Yue and Sottosanti, J Scott and Bateman, Logan and Brůža, Petr and Jiang, Shudong and Gitajn, I Leah
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Authors: Elliott, Jonathan Thomas and Henderson, Eric and Streeter, Samuel S and Demidov, Valentin and Han, Xinyue and Tang, Yue and Sottosanti, J Scott and Bateman, Logan and Brůža, Petr and Jiang, Shudong and Gitajn, I Leah
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2023)
Is the use of a routine intraoperative cholangiogram necessary in laparoscopic cholecystectomy?
Authors: Temperley, Hugo C and O'Sullivan, Niall J and Grainger, Richard and Bolger, Jarlath C
Journal: The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland (2023)
Authors: Temperley, Hugo C and O'Sullivan, Niall J and Grainger, Richard and Bolger, Jarlath C
Journal: The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland (2023)
Importance of intraoperative indocyanine green imaging in the management of non-occlusive mesenteric ischemia: a case report.
Authors: Miyashita, Ryohei and Kitazawa, Masato and Tokumaru, Shigeo and Nakamura, Satoshi and Koyama, Makoto and Yamamoto, Yuta and Hondo, Nao and Miyazaki, Satoru and Soejima, Yuji
Journal: Surgical case reports (2023): 31
Authors: Miyashita, Ryohei and Kitazawa, Masato and Tokumaru, Shigeo and Nakamura, Satoshi and Koyama, Makoto and Yamamoto, Yuta and Hondo, Nao and Miyazaki, Satoru and Soejima, Yuji
Journal: Surgical case reports (2023): 31
A novel technique proposition for determining the resection margins in lung resection by using a thermal camera.
Authors: Sayan, Muhammet and Kankoc, Aykut and Valiyev, Elgun and Celik, Ali
Journal: General thoracic and cardiovascular surgery (2023)
Authors: Sayan, Muhammet and Kankoc, Aykut and Valiyev, Elgun and Celik, Ali
Journal: General thoracic and cardiovascular surgery (2023)
Current and Future Applications of Fluorescence Guidance in Orthopaedic Surgery.
Authors: Streeter, Samuel S and Hebert, Kendra A and Bateman, Logan M and Ray, Gabrielle S and Dean, Ryan E and Geffken, Kurt T and Resnick, Corey T and Austin, Daniel C and Bell, John-Erik and Sparks, Michael B and Gibbs, Summer L and Samkoe, Kimberley S and Gitajn, I Leah and Elliott, Jonathan Thomas and Henderson, Eric R
Journal: Molecular imaging and biology (2023): 46-57
Authors: Streeter, Samuel S and Hebert, Kendra A and Bateman, Logan M and Ray, Gabrielle S and Dean, Ryan E and Geffken, Kurt T and Resnick, Corey T and Austin, Daniel C and Bell, John-Erik and Sparks, Michael B and Gibbs, Summer L and Samkoe, Kimberley S and Gitajn, I Leah and Elliott, Jonathan Thomas and Henderson, Eric R
Journal: Molecular imaging and biology (2023): 46-57