Tide Quencher™ 8WS amine [TQ8WS amine]
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS amine is primarily used for labeling biomolecules that contain a carbonyl group. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Product family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS amine [TQ4WS amine] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS amine [TQ5WS amine] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS amine [TQ6WS amine] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS amine [TQ7WS amine] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 amine [TQ1 amine] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 amine [TQ2 amine] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 amine [TQ3 amine] | 22000 | 0.085 | 0.091 |
Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission* | 250000 | - | 0.109 |
References
View all 19 references: Citation Explorer
Test method for evaluating the photocytotoxic potential of fluorescence imaging products.
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker.
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Comparison of Five Near-Infrared Fluorescent Folate Conjugates in an Ovarian Cancer Model.
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Tumor-Specific Imaging with Angiostamp800 or Bevacizumab-IRDye 800CW Improves Fluorescence-Guided Surgery over Indocyanine Green in Peritoneal Carcinomatosis.
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Page updated on November 3, 2024