Tide Quencher™ 8WS amine [TQ8WS amine]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1034.01 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 8WS maleimide [TQ8WS maleimide] |
Tide Quencher™ 8WS acid [TQ8WS acid] |
Tide Quencher™ 8WS azide [TQ8WS azide] |
Tide Quencher™ 8WS alkyne [TQ8WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1034.01 |
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS amine is primarily used for labeling biomolecules that contain a carbonyl group. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 8WS amine [TQ8WS amine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 96.711 µL | 483.554 µL | 967.109 µL | 4.836 mL | 9.671 mL |
5 mM | 19.342 µL | 96.711 µL | 193.422 µL | 967.109 µL | 1.934 mL |
10 mM | 9.671 µL | 48.355 µL | 96.711 µL | 483.554 µL | 967.109 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 4WS amine [TQ4WS amine] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS amine [TQ5WS amine] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS amine [TQ6WS amine] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS amine [TQ7WS amine] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 amine [TQ1 amine] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 amine [TQ2 amine] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3 amine [TQ3 amine] | 22000 | 0.085 | 0.091 |
Tide Fluor™ 8WS amine [TF8WS amine] *Near Infrared Emission* | 250000 | - | 0.109 |
Images
References
View all 19 references: Citation Explorer
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker.
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Comparison of Five Near-Infrared Fluorescent Folate Conjugates in an Ovarian Cancer Model.
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Authors: García de Jalón, Elvira and Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and Bjørge, Line and Haug, Bengt Erik and McCormack, Emmet
Journal: Molecular imaging and biology (2023): 144-155
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Test method for evaluating the photocytotoxic potential of fluorescence imaging products.
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Tumor-Specific Imaging with Angiostamp800 or Bevacizumab-IRDye 800CW Improves Fluorescence-Guided Surgery over Indocyanine Green in Peritoneal Carcinomatosis.
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Dual-agent fluorescent labeling of soft-tissue sarcomas improves the contrast based upon targeting both interstitial and cellular components of the tumor milieu.
Authors: Sardar, Hira S and Zai, Qais and Xu, Xiaochun and Gunn, Jason R and Pogue, Brian W and Paulsen, Keith D and Henderson, Eric R and Samkoe, Kimberley S
Journal: Journal of surgical oncology (2020): 1711-1720
Authors: Sardar, Hira S and Zai, Qais and Xu, Xiaochun and Gunn, Jason R and Pogue, Brian W and Paulsen, Keith D and Henderson, Eric R and Samkoe, Kimberley S
Journal: Journal of surgical oncology (2020): 1711-1720
Paired-Agent Fluorescence Molecular Imaging of Sentinel Lymph Nodes Using Indocyanine Green as a Control Agent for Antibody-Based Targeted Agents.
Authors: Li, Chengyue and Xu, Xiaochun and McMahon, Nathan and Alhaj Ibrahim, Omar and Sattar, Husain A and Tichauer, Kenneth M
Journal: Contrast media & molecular imaging (2019): 7561862
Authors: Li, Chengyue and Xu, Xiaochun and McMahon, Nathan and Alhaj Ibrahim, Omar and Sattar, Husain A and Tichauer, Kenneth M
Journal: Contrast media & molecular imaging (2019): 7561862
Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green.
Authors: Carr, Jessica A and Franke, Daniel and Caram, Justin R and Perkinson, Collin F and Saif, Mari and Askoxylakis, Vasileios and Datta, Meenal and Fukumura, Dai and Jain, Rakesh K and Bawendi, Moungi G and Bruns, Oliver T
Journal: Proceedings of the National Academy of Sciences of the United States of America (2018): 4465-4470
Authors: Carr, Jessica A and Franke, Daniel and Caram, Justin R and Perkinson, Collin F and Saif, Mari and Askoxylakis, Vasileios and Datta, Meenal and Fukumura, Dai and Jain, Rakesh K and Bawendi, Moungi G and Bruns, Oliver T
Journal: Proceedings of the National Academy of Sciences of the United States of America (2018): 4465-4470