Tide Quencher™ 8WS maleimide [TQ8WS maleimide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1000.03 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
Tide Quencher™ 8WS amine [TQ8WS amine] |
Tide Quencher™ 8WS acid [TQ8WS acid] |
Tide Quencher™ 8WS azide [TQ8WS azide] |
Tide Quencher™ 8WS alkyne [TQ8WS alkyne] |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 1000.03 |
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS maleimide is primarily used for the post-labeling of thiol-modified oligonucleotides and the Cys residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 8WS maleimide [TQ8WS maleimide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 99.997 µL | 499.985 µL | 999.97 µL | 5 mL | 10 mL |
5 mM | 19.999 µL | 99.997 µL | 199.994 µL | 999.97 µL | 2 mL |
10 mM | 10 µL | 49.999 µL | 99.997 µL | 499.985 µL | 999.97 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 2WS maleimide [TQ2WS maleimide] | 48000 | 0.100 | 0.120 |
Tide Quencher™ 4WS maleimide [TQ4WS maleimide] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS maleimide [TQ5WS maleimide] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS maleimide [TQ6WS maleimide] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS maleimide [TQ7WS maleimide] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 maleimide [TQ1 maleimide] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 3 maleimide [TQ3 maleimide] | 22000 | 0.085 | 0.091 |
Tide Fluor™ 8WS maleimide [TF8WS maleimide] *Near Infrared Emission* | 250000 | - | 0.109 |
Images
References
View all 10 references: Citation Explorer
Performance of two clinical fluorescence imaging systems with different targeted and non-targeted near-infrared fluorophores: a cadaveric explorative study.
Authors: Chiti, Lavinia E and Husi, Benjamin and Park, Brian and Beer, Patricia and D'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Frontiers in veterinary science (2023): 1091842
Authors: Chiti, Lavinia E and Husi, Benjamin and Park, Brian and Beer, Patricia and D'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Frontiers in veterinary science (2023): 1091842
Impact of Surgical Lights on the Performance of Fluorescence-Guided Surgery Systems: A Pilot Study.
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Authors: Chiti, Lavinia E and Park, Brian and d'Orchymont, Faustine and Holland, Jason P and Nolff, Mirja C
Journal: Animals : an open access journal from MDPI (2023)
Test method for evaluating the photocytotoxic potential of fluorescence imaging products.
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Authors: Vig, Shruti and Gaitan, Brandon and Frankle, Lucas and Chen, Yu and Elespuru, Rosalie and Pfefer, T Joshua and Huang, Huang-Chiao
Journal: Photochemistry and photobiology (2023)
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Structurally symmetric near-infrared fluorophore IRDye78-protein complex enables multimodal cancer imaging.
Authors: Yang, Jiang and Zhao, Chunhua and Lim, Jacky and Zhao, Lina and Tourneau, Ryan Le and Zhang, Qize and Dobson, Damien and Joshi, Suhasini and Pang, Jiadong and Zhang, Xiaodong and Pal, Suchetan and Andreou, Chrysafis and Zhang, Hanwen and Kircher, Moritz F and Schmitthenner, Hans
Journal: Theranostics (2021): 2534-2549
Authors: Yang, Jiang and Zhao, Chunhua and Lim, Jacky and Zhao, Lina and Tourneau, Ryan Le and Zhang, Qize and Dobson, Damien and Joshi, Suhasini and Pang, Jiadong and Zhang, Xiaodong and Pal, Suchetan and Andreou, Chrysafis and Zhang, Hanwen and Kircher, Moritz F and Schmitthenner, Hans
Journal: Theranostics (2021): 2534-2549
A mini-review on rare-earth down-conversion nanoparticles for NIR-II imaging of biological systems.
Authors: Zhong, Yeteng and Dai, Hongjie
Journal: Nano research (2020): 1281-1294
Authors: Zhong, Yeteng and Dai, Hongjie
Journal: Nano research (2020): 1281-1294
Near-Infrared-II (NIR-II) Bioimaging via Off-Peak NIR-I Fluorescence Emission.
Authors: Zhu, Shoujun and Yung, Bryant C and Chandra, Swati and Niu, Gang and Antaris, Alexander L and Chen, Xiaoyuan
Journal: Theranostics (2018): 4141-4151
Authors: Zhu, Shoujun and Yung, Bryant C and Chandra, Swati and Niu, Gang and Antaris, Alexander L and Chen, Xiaoyuan
Journal: Theranostics (2018): 4141-4151
Near-infrared fluorescence laparoscopy of the cystic duct and cystic artery: first experience with two new preclinical dyes in a pig model.
Authors: van den Bos, Jacqueline and Al-Taher, Mahdi and Hsien, Shu Gi and Bouvy, Nicole D and Stassen, Laurents P S
Journal: Surgical endoscopy (2017): 4309-4314
Authors: van den Bos, Jacqueline and Al-Taher, Mahdi and Hsien, Shu Gi and Bouvy, Nicole D and Stassen, Laurents P S
Journal: Surgical endoscopy (2017): 4309-4314
Non-invasive optical imaging of the lymphatic vasculature of a mouse.
Authors: Robinson, Holly A and Kwon, SunKuk and Hall, Mary A and Rasmussen, John C and Aldrich, Melissa B and Sevick-Muraca, Eva M
Journal: Journal of visualized experiments : JoVE (2013): e4326
Authors: Robinson, Holly A and Kwon, SunKuk and Hall, Mary A and Rasmussen, John C and Aldrich, Melissa B and Sevick-Muraca, Eva M
Journal: Journal of visualized experiments : JoVE (2013): e4326
Real-time intraoperative assessment of the extrahepatic bile ducts in rats and pigs using invisible near-infrared fluorescent light.
Authors: Tanaka, Eiichi and Choi, Hak Soo and Humblet, Valerie and Ohnishi, Shunsuke and Laurence, Rita G and Frangioni, John V
Journal: Surgery (2008): 39-48
Authors: Tanaka, Eiichi and Choi, Hak Soo and Humblet, Valerie and Ohnishi, Shunsuke and Laurence, Rita G and Frangioni, John V
Journal: Surgery (2008): 39-48