logo
AAT Bioquest

Tide Quencher™ 8WS succinimidyl ester [TQ8WS SE]

Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS succinimidyl ester is primarily used for the post-labeling of amino-modified oligonucleotides and the N-terminal or lysine residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.

Spectrum

References

View all 21 references: Citation Explorer
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker.
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Tumor-Specific Imaging with Angiostamp800 or Bevacizumab-IRDye 800CW Improves Fluorescence-Guided Surgery over Indocyanine Green in Peritoneal Carcinomatosis.
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Near infrared fluorescence imaging of the urethra: a systematic review of the literature.
Authors: Al-Taher, Mahdi and Knapen, Bob and Barberio, Manuel and Felli, Eric and Gioux, Sylvain and Bouvy, Nicole D and Stassen, Laurents P S and Marescaux, Jacques and Diana, Michele
Journal: Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy (2022): 342-349
Simultaneous multipurpose fluorescence imaging with IRDye® 800BK during laparoscopic surgery.
Authors: Al-Taher, Mahdi and Barberio, Manuel and Felli, Eric and Agnus, Vincent and Ashoka, Anila Hoskere and Gioux, Sylvain and Klymchenko, Andrey and Bouvy, Nicole and Stassen, Laurents and Marescaux, Jacques and Diana, Michele
Journal: Surgical endoscopy (2021): 4840-4848
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Page updated on October 4, 2024

Ordering information

Price
Unit size
Catalog Number2135
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

962.15

Solvent

DMSO

Spectral properties

Absorbance (nm)

881

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
Product Image
Product Image
Gallery Image 1