Tide Quencher™ 8WS succinimidyl ester [TQ8WS SE]
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 962.15 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | ![]() ![]() |
See also: Tide Quencher Dyes
Molecular weight 962.15 |
Tide Quencher™ 8WS (TQ8WS) is a non-fluorescent molecule designed to efficiently quench the fluorescence of IR fluorophores such as ICG, iFluor® 820, iFluor® 840 and iFluor® 860. It has the longest absorption wavelength among all the commercial quenchers. TQ8WS is designed to be the most effective IR quencher with (a). much stronger absorption, and (b). much higher quenching efficiency for IR dyes. Tide Quencher™ 8WS succinimidyl ester is primarily used for the post-labeling of amino-modified oligonucleotides and the N-terminal or lysine residues of peptides. It can be used in techniques such as polymerase chain reaction (PCR), real-time PCR, and DNA sequencing. In these applications, fluorescence signals are used to monitor the amplification or detection of specific DNA sequences. TQ8WS quenches the fluorescent signal until a specific event (like DNA strand separation or primer extension) occurs, leading to an increase in fluorescence that can be detected and quantified. Fluorescence resonance energy transfer (FRET)-based assays are widely used to detect and measure the presence of specific molecules in a sample. They involve the use of a fluorescent molecule (fluorophore) and a quencher molecule such as TQ8WS. The fluorophore emits light when excited by a specific wavelength of light, while the quencher molecule absorbs this emitted light, effectively "quenching" the fluorescence signal.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Tide Quencher™ 8WS succinimidyl ester [TQ8WS SE] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 103.934 µL | 519.669 µL | 1.039 mL | 5.197 mL | 10.393 mL |
5 mM | 20.787 µL | 103.934 µL | 207.868 µL | 1.039 mL | 2.079 mL |
10 mM | 10.393 µL | 51.967 µL | 103.934 µL | 519.669 µL | 1.039 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
Tide Quencher™ 2WS succinimidyl ester [TQ2WS, SE] | 48000 | 0.100 | 0.120 |
Tide Quencher™ 4WS succinimidyl ester [TQ4WS SE] | 900001 | 0.149 | 0.136 |
Tide Quencher™ 5WS succinimidyl ester [TQ5WS SE] | 130000 | 0.072 | 0.082 |
Tide Quencher™ 6WS succinimidyl ester [TQ6WS SE] | 130000 | 0.120 | 0.102 |
Tide Quencher™ 7WS succinimidyl ester [TQ7WS SE] | 140000 | 0.072 | 0.091 |
Tide Quencher™ 1 succinimidyl ester [TQ1 SE] | 20000 | 0.147 | 0.194 |
Tide Quencher™ 2 succinimidyl ester [TQ2 SE] | 21000 | 0.100 | 0.12 |
Tide Quencher™ 3WS succinimidyl ester [TQ3WS SE] | 90000 | 0.186 | 0.205 |
Tide Quencher™ 3 succinimidyl ester [TQ3 SE] | 22000 | 0.085 | 0.091 |
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Images
References
View all 21 references: Citation Explorer
Comparison of Near-Infrared Imaging Agents Targeting the PTPmu Tumor Biomarker.
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Authors: Johansen, Mette L and Vincent, Jason and Rose, Marissa and Sloan, Andrew E and Brady-Kalnay, Susann M
Journal: Molecular imaging and biology (2023): 744-757
Tumor-Specific Imaging with Angiostamp800 or Bevacizumab-IRDye 800CW Improves Fluorescence-Guided Surgery over Indocyanine Green in Peritoneal Carcinomatosis.
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Authors: Josserand, Véronique and Bernard, Claire and Michy, Thierry and Guidetti, Mélanie and Vollaire, Julien and Coll, Jean-Luc and Hurbin, Amandine
Journal: Biomedicines (2022)
Near infrared fluorescence imaging of the urethra: a systematic review of the literature.
Authors: Al-Taher, Mahdi and Knapen, Bob and Barberio, Manuel and Felli, Eric and Gioux, Sylvain and Bouvy, Nicole D and Stassen, Laurents P S and Marescaux, Jacques and Diana, Michele
Journal: Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy (2022): 342-349
Authors: Al-Taher, Mahdi and Knapen, Bob and Barberio, Manuel and Felli, Eric and Gioux, Sylvain and Bouvy, Nicole D and Stassen, Laurents P S and Marescaux, Jacques and Diana, Michele
Journal: Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy (2022): 342-349
Deep learning for in vivo near-infrared imaging.
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Simultaneous multipurpose fluorescence imaging with IRDye® 800BK during laparoscopic surgery.
Authors: Al-Taher, Mahdi and Barberio, Manuel and Felli, Eric and Agnus, Vincent and Ashoka, Anila Hoskere and Gioux, Sylvain and Klymchenko, Andrey and Bouvy, Nicole and Stassen, Laurents and Marescaux, Jacques and Diana, Michele
Journal: Surgical endoscopy (2021): 4840-4848
Authors: Al-Taher, Mahdi and Barberio, Manuel and Felli, Eric and Agnus, Vincent and Ashoka, Anila Hoskere and Gioux, Sylvain and Klymchenko, Andrey and Bouvy, Nicole and Stassen, Laurents and Marescaux, Jacques and Diana, Michele
Journal: Surgical endoscopy (2021): 4840-4848
Tumor-specific near-infrared nanobody probe rapidly labels tumors in an orthotopic mouse model of pancreatic cancer.
Authors: Lwin, Thinzar M and Hernot, Sophie and Hollandsworth, Hannah and Amirfakhri, Siamak and Filemoni, Filemoni and Debie, Pieterjan and Hoffman, Robert M and Bouvet, Michael
Journal: Surgery (2020): 85-91
Authors: Lwin, Thinzar M and Hernot, Sophie and Hollandsworth, Hannah and Amirfakhri, Siamak and Filemoni, Filemoni and Debie, Pieterjan and Hoffman, Robert M and Bouvet, Michael
Journal: Surgery (2020): 85-91
Dual-agent fluorescent labeling of soft-tissue sarcomas improves the contrast based upon targeting both interstitial and cellular components of the tumor milieu.
Authors: Sardar, Hira S and Zai, Qais and Xu, Xiaochun and Gunn, Jason R and Pogue, Brian W and Paulsen, Keith D and Henderson, Eric R and Samkoe, Kimberley S
Journal: Journal of surgical oncology (2020): 1711-1720
Authors: Sardar, Hira S and Zai, Qais and Xu, Xiaochun and Gunn, Jason R and Pogue, Brian W and Paulsen, Keith D and Henderson, Eric R and Samkoe, Kimberley S
Journal: Journal of surgical oncology (2020): 1711-1720
Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging.
Authors: Fuenzalida Werner, Juan Pablo and Huang, Yuanhui and Mishra, Kanuj and Janowski, Robert and Vetschera, Paul and Heichler, Christina and Chmyrov, Andriy and Neufert, Clemens and Niessing, Dierk and Ntziachristos, Vasilis and Stiel, Andre C
Journal: Analytical chemistry (2020)
Authors: Fuenzalida Werner, Juan Pablo and Huang, Yuanhui and Mishra, Kanuj and Janowski, Robert and Vetschera, Paul and Heichler, Christina and Chmyrov, Andriy and Neufert, Clemens and Niessing, Dierk and Ntziachristos, Vasilis and Stiel, Andre C
Journal: Analytical chemistry (2020)
Paired-Agent Fluorescence Molecular Imaging of Sentinel Lymph Nodes Using Indocyanine Green as a Control Agent for Antibody-Based Targeted Agents.
Authors: Li, Chengyue and Xu, Xiaochun and McMahon, Nathan and Alhaj Ibrahim, Omar and Sattar, Husain A and Tichauer, Kenneth M
Journal: Contrast media & molecular imaging (2019): 7561862
Authors: Li, Chengyue and Xu, Xiaochun and McMahon, Nathan and Alhaj Ibrahim, Omar and Sattar, Husain A and Tichauer, Kenneth M
Journal: Contrast media & molecular imaging (2019): 7561862
Structure-activity relationships of succinimidyl-Cys-C(O)-Glu derivatives with different near-infrared fluorophores as optical imaging probes for prostate-specific membrane antigen.
Authors: Matsuoka, Daiko and Watanabe, Hiroyuki and Shimizu, Yoichi and Kimura, Hiroyuki and Yagi, Yusuke and Kawai, Ryoko and Ono, Masahiro and Saji, Hideo
Journal: Bioorganic & medicinal chemistry (2018): 2291-2301
Authors: Matsuoka, Daiko and Watanabe, Hiroyuki and Shimizu, Yoichi and Kimura, Hiroyuki and Yagi, Yusuke and Kawai, Ryoko and Ono, Masahiro and Saji, Hideo
Journal: Bioorganic & medicinal chemistry (2018): 2291-2301