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TMA-DPH *CAS#: 115534-33-3*

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Physical properties
Molecular weight461.62
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
115534-33-3
Molecular weight
461.62
TMA-DPH is a fluorescent probe for membrane fluidity measurements. It is especially useful in the study of monolayer dynamics of lipoproteins and similar analogous systems. DPH and its derivatives (such as TMA-DPH) are cylinder-shaped and have fluorescence emission transition dipoles that are basically aligned parallel to their long molecular axis. As such, they are very sensitive to reorientation resulting from interactions with surrounding lipids. They are widely used in fluorescence polarization studies of rotational motion.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of TMA-DPH *CAS#: 115534-33-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM216.628 µL1.083 mL2.166 mL10.831 mL21.663 mL
5 mM43.326 µL216.628 µL433.257 µL2.166 mL4.333 mL
10 mM21.663 µL108.314 µL216.628 µL1.083 mL2.166 mL

Molarity calculator

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Citations


View all 10 citations: Citation Explorer
Toxicity of hexagonal boron nitride nanosheets to freshwater algae: Phospholipid membrane damage and carbon assimilation inhibition
Authors: Zou, Wei and Huo, Yuhan and Zhang, Xingli and Jin, Caixia and Li, Xiaokang and Cao, Zhiguo
Journal: Journal of Hazardous Materials (2023): 133204
Cis-and trans-variations of Stearoyl-CoA Desaturase Provide New Insights into the Mechanisms of Diverged Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species
Authors: Wang, Chaogang and Li, Ao and Cong, Rihao and Qi, Haigang and Wang, Wei and Zhang, Guofan and Li, Li
Journal: Molecular Biology and Evolution (2023)
Size-dependent effects of polystyrene microplastics on cytotoxicity and efflux pump inhibition in human Caco-2 cells
Authors: Wu, Bing and Wu, Xiaomei and Liu, Su and Wang, Zhizhi and Chen, Ling
Journal: Chemosphere (2019)
Comparison of cytotoxicity and membrane efflux pump inhibition in HepG2 cells induced by single-walled carbon nanotubes with different length and functional groups
Authors: Shen, Zhuoyan and Wu, Jialu and Yu, Yue and Liu, Su and Jiang, Wei and Nurmamat, Habiba and Wu, Bing
Journal: Scientific Reports (2019): 7557
Interaction of Hsp90 with phospholipid model membranes
Authors: Zhang, Muhan and Wang, Daoying and Li, Pengpeng and Sun, Chong and Xu, Rong and Geng, Zhiming and Xu, Weimin and Dai, Zhaoqi
Journal: Biochimica et Biophysica Acta (BBA)-Biomembranes (2018): 611--616
Interaction of Hsp90 with phospholipid model membranes
Authors: Zhang, Muhan and Wang, Daoying and Li, Pengpeng and Sun, Chong and Xu, Rong and Geng, Zhiming and Xu, Weimin and Dai, Zhaoqi
Journal: Biochimica et Biophysica Acta (BBA)-Biomembranes (2017)
Cytotoxicity and efflux pump inhibition induced by molybdenum disulfide and boron nitride nanomaterials with sheetlike structure
Authors: Liu, Su and Shen, Zhuoyan and Wu, Bing and Yu, Yue and Hou, Hui and Zhang, Xu-Xiang and Ren, Hong-qiang
Journal: Environmental Science \& Technology (2017): 10834--10842
Cytotoxicity and efflux pump inhibition induced by molybdenum disulfide and boron nitride nanomaterials with sheet-like structure
Authors: Liu, Su and Shen, Zhuoyan and Wu, Bing and Yu, Yue and Hou, Hui and Zhang, Xu-Xiang and Ren, Hong-qiang
Journal: Environmental Science &amp; Technology (2017)
Comparison of Cytotoxicity and Inhibition of Membrane ABC Transporters Induced by MWCNTs with Different Length and Functional Groups
Authors: Yu, Jing and Liu, Su and Wu, Bing and Shen, Zhuoyan and Cherr, Gary N and Zhang, Xu-Xiang and Li, Mei
Journal: Environmental science &amp; technology (2016): 3985--3994
Low levels of graphene and graphene oxide inhibit cellular xenobiotic defense system mediated by efflux transporters
Authors: Liu, Su and Jiang, Wei and Wu, Bing and Yu, Jing and Yu, Haiyan and Zhang, Xu-Xiang and Torres-Duarte, Cristina and Cherr, Gary N
Journal: Nanotoxicology (2016): 597--606

References


View all 16 references: Citation Explorer
Fluorescent probes DPH, TMA-DPH and C17-HC induce erythrocyte exovesiculation
Authors: Saldanha C, Santos NC, Martins-Silva J.
Journal: J Membr Biol (2002): 75
Fluorescent lipid probes 12-AS and TMA-DPH report on selective, purinergically induced fluidity changes in plasma membranes of lymphoid cells
Authors: Matko J, Nagy P.
Journal: J Photochem Photobiol B (1997): 120
Plasma membrane fluidity of keratinocytes of normal and psoriatic skin: a study using fluorescence anisotropy of trimethylammoniumdiphenylhexatriene (TMA-DPH)
Authors: Simonetti O, Ferretti G, Offidani AM, Gervasi P, Curatola G, Bossi G.
Journal: Arch Dermatol Res (1996): 51
Enzymically generated electron-excited species increase the membrane order parameter without changing the rotational relaxation time of TMA-DPH
Authors: Obsil T, Amler E, Kotyk A, Pavlicek Z.
Journal: Gen Physiol Biophys (1995): 393
A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane
Authors: Illinger D, Duportail G, Mely Y, Poirel-Morales N, Gerard D, Kuhry JG.
Journal: Biochim Biophys Acta (1995): 58
The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells
Authors: Illinger D, Kuhry JG.
Journal: J Cell Biol (1994): 783
Time-resolved polarized fluorescence studies of the temperature adaptation in Bacillus subtilis using DPH and TMA-DPH fluorescent probes
Authors: Herman P, Konopasek I, Plasek J, Svobodova J.
Journal: Biochim Biophys Acta (1994): 1
Membrane heterogeneity in isolated rat hepatocytes and liver plasma membrane subfractions: a comparative study using DPH and its cationic derivative TMA-DPH
Authors: Ferretti G, Zolese G, Curatola G, Jezequel AM, Benedetti A.
Journal: Biochim Biophys Acta (1993): 245
Properties of a phosphatidylcholine derivative of diphenyl hexatriene (DPH-PC) in lymphocyte membranes. A comparison with DPH and the cationic derivative TMA-DPH using static and dynamic fluorescence
Authors: Ferretti G, Tangorra A, Zolese G, Curatola G.
Journal: Membr Biochem (1993): 17
Comparative evolution of endocytosis levels and of the cell surface area during the L929 cell cycle: a fluorescence study with TMA-DPH
Authors: Illinger D, Italiano L, Beck JP, Waltzinger C, Kuhry JG.
Journal: Biol Cell (1993): 265