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Transfectamine™ 5000 Transfection Reagent
Transfectamine™ 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. It can effectively transfect a variety of payloads into a variety of adherent and suspension cell lines. It can be used for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies. It offers consistently high transfection efficiency in a wide variety of adherent and suspension cell lines, including difficult-to-transfect cells. The low toxicity of Transfectamine™ 5000 also allowed higher viability of transfected cells. Transfectamine™ 5000 is easier to use compare to most other transfection reagents and does not require special medium.
Transfection efficiency comparison in HeLa cells using Transfectamine™ 5000, Lipofectamine 2000 and Lipofectamine 3000 reagents. Each reagent was used to transfect HeLa cells in a 96-well format, and GFP expression was analyzed 24 hours post-transfection. Transfectamine™ 5000 transfection reagent provided higher GFP transfection efficiency compared to Lipofectamine 2000 and Lipofectamine 3000 reagents.
Transfection efficiency comparison in HeLa cells using Transfectamine™ 5000, Lipofectamine 2000 and Lipofectamine 3000 reagents. Each reagent was used to transfect HeLa cells in a 96-well format, and GFP expression was analyzed 24 hours post-transfection. Transfectamine™ 5000 transfection reagent provided higher GFP transfection efficiency compared to Lipofectamine 2000 and Lipofectamine 3000 reagents.
CatalogSize
Price
Quantity
6001950 uL
Price
600200.5 mL
Price
600211 mL
Price
600225 mL
Price
 
Physical properties

SolventWater
Storage, safety and handling

Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on September 21, 2025