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Transfectamine™ 6000 CRISPR Transfection Reagent
Transfectamine™ 6000 CRISPR Transfection Reagent is a non-liposomal formula designed for optimal delivery and effectiveness of CRISPR/Cas9 components across a wide array of cell types. Engineered for targeted genome editing, this reagent demonstrates superior transfection efficiency, particularly in cell lines typically resistant to transfection. The versatility of Transfectamine™ 6000 CRISPR Transfection Reagent supports various CRISPR/Cas9 systems, including plasmids, ribonucleoproteins (RNPs), and mRNA. Its formulation has been optimized to minimize cytotoxicity while maintaining cell viability, ensuring more accurate and reliable experimental results. The straightforward protocol associated with our reagent reduces the need for extensive optimization, streamlining the workflow and conserving valuable time and resources. Transfectamine™ 6000 CRISPR Transfection Reagent is suitable for various applications, such as gene knockout and knock-in experiments, the generation of CRISPR-mediated gene-edited cell lines, functional genomics studies, and research in therapeutic gene editing.
Comparison of transfection efficiency in HeLa cells for CRISPR-Cas9-GFP plasmid using Transfectamine™ 6000 CRISPR Transfection Reagent and Lipofectamine 2000. Both reagents were used to transfect HeLa cells in a 96-well format, and GFP expression was analyzed 48 and 72 hours post-transfection. Transfectamine™ 6000 CRISPR Transfection reagent provided higher GFP transfection efficiency than Lipofectamine 2000.
Comparison of transfection efficiency in HeLa cells for CRISPR-Cas9-GFP plasmid using Transfectamine™ 6000 CRISPR Transfection Reagent and Lipofectamine 2000. Both reagents were used to transfect HeLa cells in a 96-well format, and GFP expression was analyzed 48 and 72 hours post-transfection. Transfectamine™ 6000 CRISPR Transfection reagent provided higher GFP transfection efficiency than Lipofectamine 2000.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
600231 mL
Price
600245 mL
Price
6002850 uL
Price
 
Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 4, 2025