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Transfectamine™ 7000 siRNA Transfection Reagent
Transfectamine™ 7000 siRNA Transfection Reagent provides a reliable and effective solution for siRNA-mediated gene knockdown experiments, efficiently delivering small interfering RNA (siRNA) and microRNA (miRNA) into diverse cell types. This reagent allows researchers to achieve high transfection efficiency with lower RNAi concentrations, resulting in effective gene knockdown with minimal non-specific effects. The low cytotoxicity of Transfectamine™ 7000 facilitates easy optimization across various concentration ranges, ensuring minimal impact on cell viability. The application protocol for Transfectamine™ 7000 is streamlined and rapid, promoting consistent and reproducible outcomes in gene silencing experiments. This reagent is capable of achieving significant levels of gene knockdown in a broad spectrum of cell lines, which is crucial for obtaining robust and conclusive experimental data. For high-throughput siRNA transfection applications, Transfectamine™ 7000 offers a straightforward workflow: the reagent is mixed with siRNA, applied to cells, incubated, and then the extent of gene knockdown is assessed. The efficiency and speed of this process, combined with the high transfection efficiency, makes Transfectamine™ 7000 an optimal choice for high-throughput screening, and it can be readily adapted for automated or robotic systems.
HeLa cells were subjected to immunohistochemistry after transfecting with control siRNA or GAPDH siRNA using Lipofectamine 2000 and Transfectamine™ 7000. The experiment aimed to compare the efficacy of both transfection methods in knocking down GAPDH. After 48 hours of transfection, GAPDH was detected using an iFluor® 488 goat anti-mouse IgG (Cat No. 16528) through immunohistochemistry.
HeLa cells were subjected to immunohistochemistry after transfecting with control siRNA or GAPDH siRNA using Lipofectamine 2000 and Transfectamine™ 7000. The experiment aimed to compare the efficacy of both transfection methods in knocking down GAPDH. After 48 hours of transfection, GAPDH was detected using an iFluor® 488 goat anti-mouse IgG (Cat No. 16528) through immunohistochemistry.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
600251 mL
Price
600265 mL
Price
6002750 uL
Price
 
Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 26, 2025