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Transfectamine™ mRNA Transfection Reagent
Transfectamine™ mRNA Transfection Reagent is a powerful and versatile transfection reagent designed to introduce a higher amount of mRNA into eukaryotic cells, or more specifically, into animal cells. It delivers high transfection efficiency in a wide variety of adherent and suspension cell lines, including difficult-to-transfect cells. Nuclear uptake is not required, which results in faster protein expression than DNA transfection without the risk of genomic integration. The low toxicity of Transfectamine™ mRNA Transfection Reagent allows higher viability of transfected cells. Transfectamine™ mRNA Transfection Reagent does not require special medium and is easier to use compared to most of the commercial transfection reagents.
Transfection efficiency comparison (Upper panel) and cellular toxicity comparison (Bottom panel) in HeLa cells. HeLa cells were cultured in a 6-well plate to ~90% confluency. 2.5 µg of mRNA was transfected with Lipofectamine MessengerMAX and Transfectamine™ mRNA Transfection Reagent, respectively. Images were taken 18 hours after the transfection using a fluorescent microscope with the FITC channel (Upper panel). Although transfection efficiency was similar for Lipofectamine MessengerMAX and Transfectamine™ mRNA Transfection Reagent, most Lipofectamine MessengerMAX transfected samples were scrambled, whereas cells transfected with Transfectamine™ mRNA Transfection Reagent looked much healthier (bottom panel).
Transfection efficiency comparison (Upper panel) and cellular toxicity comparison (Bottom panel) in HeLa cells. HeLa cells were cultured in a 6-well plate to ~90% confluency. 2.5 µg of mRNA was transfected with Lipofectamine MessengerMAX and Transfectamine™ mRNA Transfection Reagent, respectively. Images were taken 18 hours after the transfection using a fluorescent microscope with the FITC channel (Upper panel). Although transfection efficiency was similar for Lipofectamine MessengerMAX and Transfectamine™ mRNA Transfection Reagent, most Lipofectamine MessengerMAX transfected samples were scrambled, whereas cells transfected with Transfectamine™ mRNA Transfection Reagent looked much healthier (bottom panel).
CatalogSize
Price
Quantity
6002950 uL
Price
600300.5 mL
Price
600315 mL
Price
 
Physical properties

SolventWater
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 4, 2025