Transfectamine™ mRNA Transfection Reagent
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Example protocol
AT A GLANCE
Prepare cells for transfection.
Prepare the Transfectamine™ mRNA Transfection Reagent-RNA mixture.
Add the Transfectamine™ mRNA Transfection Reagent-RNA mixture to the cell culture.
Culture cells overnight.
Analyze transfection efficiency with an appropriate method.
CELL PREPARATION
Culture cells to ~ 90% confluency at the time of transfection.
Replace with fresh growth medium before transfection. For example, replace with 2 mL of medium per well for 6-well plates and 6 mL of medium for 10 cm plates.
PREPARATION OF WORKING SOLUTION
Mix 2.5 µg of mRNA with 200 µL of serum-free medium.
Add 7.5 µL of Transfectamine™ mRNA Transfection Reagent to Step 1.
Mix well and incubate at RT for 20 minutes.
Note: The ratio of Transfectamine™ mRNA Transfection Reagent to mRNA needs to be optimized for different cell lines. In general, Transfectamine™ mRNA Transfection Reagent (µL) to mRNA (µg) Ratio = (3 to 5 µL) to 1 µg.
Table 1. Sample protocol detail for 6-well plates as shown in the table below.
Component | 6-well plate (per well) |
Fresh culture medium | 2 mL |
Purified mRNA | ~2.5 µg |
Serum-free medium | 200 µL |
Transfectamine™ mRNA Transfection Reagent | ~7.5 µL |
SAMPLE EXPERIMENTAL PROTOCOL
Add the Transfectamine™ mRNA Transfection Reagent -mRNA mixture to the culture plate and culture overnight.
Note: Recombinant protein expression can be detected as early as 8 hours after the transfection. Maximal expression level may be observed ~24 hours after the transfection.
Images
References
Authors: Lin, Liang and Cho, Shih-Feng and Xing, Lijie and Wen, Kenneth and Li, Yuyin and Yu, Tengteng and Hsieh, Phillip A and Chen, Hailin and Kurtoglu, Metin and Zhang, Yi and Andrew Stewart, C and Munshi, Nikhil and Anderson, Kenneth C and Tai, Yu-Tzu
Journal: Leukemia (2021): 752-763
Authors: Zhang, Wei and Liu, Yi and Min Chin, Jas and Phua, Kyle K L
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2021): 179-187
Authors: Alvarez-Palomo, Ana Belén and Requena-Osete, Jordi and Delgado-Morales, Raul and Moreno-Manzano, Victoria and Grau-Bove, Carme and Tejera, Agueda M and Otero, Manel Juan and Barrot, Carme and Santos-Barriopedro, Irene and Vaquero, Alejandro and Mezquita-Pla, Jovita and Moran, Sebastian and Naya, Carlos Hobeich and Garcia-Martínez, Iris and Pérez, Francisco Vidal and Blasco, María A and Esteller, Manel and Edel, Michael J
Journal: Stem cells (Dayton, Ohio) (2021)
Authors: Dawicki, Wojciech and Huang, Hui and Ma, Yanna and Town, Jennifer and Zhang, Xiaobei and Rudulier, Chris D and Gordon, John R
Journal: PloS one (2021): e0248290
Authors: Schwarze, Lea-Isabell and Fehse, Boris
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 217-226
Authors: Lee, Sang M and Cheng, Qiang and Yu, Xueliang and Liu, Shuai and Johnson, Lindsay T and Siegwart, Daniel J
Journal: Angewandte Chemie (International ed. in English) (2021): 5848-5853
Authors: Vigil, Toriana N and Zhang-Hulsey, Diana and Santos, Jose Luis and Patrick Hussmann, G
Journal: Analytical biochemistry (2021): 114259
Authors: Reiser, Anita and Woschée, Daniel and Kempe, Simon Maximilian and Rädler, Joachim Oskar
Journal: Journal of visualized experiments : JoVE (2021)
Authors: He, Jiaxi and Xu, Songhui and Leng, Qixin and Mixson, A James
Journal: The journal of gene medicine (2021): e3295
Authors: Grun, Molly K and Suberi, Alexandra and Shin, Kwangsoo and Lee, Teresa and Gomerdinger, Victoria and Moscato, Zoe M and Piotrowski-Daspit, Alexandra S and Saltzman, W Mark
Journal: Biomaterials (2021): 120780
Application notes
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM