Name: trFluor™ Eu-Cryptate succinimidyl ester
Brand: AAT Bioquest
SKU: 1430
Availability: InStock

trFluor™ Eu-Cryptate succinimidyl ester

Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluor™ Cryptate Eu succinimidyl ester has the highest stability with good water solubility. Compared to the other commercial Cryptate Eu probes, trFluor™ Cryptate Eu succinimidyl ester only has a single reactive group, eliminating the crosslinking possibility. These trFluor™ Eu probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluor™ Cryptate Eu probes have extremely high stability, high emission yield and ability to selectively label biomolecules. Moreover, our trFluor™ Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.

TR-FRET assay using Streptavidin-TrFluor™ Cryptate Eu (#1431). The conjugate was prepared and TR-FRET signals were detected in the presence of Biotinylated-trFluor™ Eu Acceptor XL665 (#1440).

Protocol

COA

Catalog	Size	Price	Quantity
1430	100 ug	Price
1431	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of trFluor™ Eu-Cryptate succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	63.54 µL	317.702 µL	635.405 µL	3.177 mL	6.354 mL
5 mM	12.708 µL	63.54 µL	127.081 µL	635.405 µL	1.271 mL
10 mM	6.354 µL	31.77 µL	63.54 µL	317.702 µL	635.405 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1573.8 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Systemically-delivered biodegradable PLGA alters gut microbiota and induces transcriptomic reprogramming in the liver in an obesity mouse model

Authors:

Chaplin, Alice and Gao, Huiyun and Asase, Courteney and Rengasamy, Palanivel and Park, Bongsoo and Skander, Danielle and Bebek, G{\"u}rkan and Rajagopalan, Sanjay and Maiseyeu, Andrei

Journal:

Scientific reports (2020): 1--16

Nano-Antagonist Alleviates Inflammation and Allows for MRI of Atherosclerosis

Authors:

Mog, Brian and Asase, Courteney and Chaplin, Alice and Gao, Huiyun and Rajagopalan, Sanjay and Maiseyeu, Andrei

Journal:

Nanotheranostics (2019): 342

References

View all 61 references: Citation Explorer

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors

Authors:

Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.

Journal:

Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays

Authors:

Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.

Journal:

Assay Drug Dev Technol. (2012)

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach

Authors:

Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.

Journal:

J Phys Chem B (2011): 11439

A homogeneous single-label time-resolved fluorescence cAMP assay

Authors:

Martikkala E, Rozw and owicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.

Journal:

J Biomol Screen (2011): 356

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies

Authors:

Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.

Journal:

J Biol Chem (2011): 11337

Physical properties

Molecular weight	1573.80
Solvent	DMSO

Spectral properties

Absorbance (nm)	305
Correction factor (280 nm)	0.416
Extinction coefficient (cm ^-1 M ^-1)	28000 ¹
Excitation (nm)	305
Emission (nm)	617

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 1430)
Safety Data Sheet (Catalog 1431)
Certificate of Analysis

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Page updated on October 18, 2025