Name: Tris NTA alkyne
Brand: AAT Bioquest
SKU: 12636
Price: 765 USD
Availability: InStock

Tris NTA alkyne

Tris-NTA Alkyne is a high-affinity polyhistidine tag binder with click chemistry functionality, enabling the development of specific probes for His-tagged protein detection.

Trivalent His-Tag Chelator: Three NTA groups provide nM affinity for His-tagged proteins
Enhanced Stability vs Mono-NTA: Multivalent binding offers 1000-fold slower dissociation rates and more stable protein complexes
Click Chemistry/Bioorthogonal Handle: Alkyne group enables Cu-catalyzed azide-alkyne cycloaddition for site-specific bioconjugation
Versatile Protein Applications: Enables His-tagged protein purification, selective labeling in cell lysates, and live-cell surface protein visualization

The chemical structure of Tris NTA alkyne.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
12636	1 mg	Price

Overview

Tris NTA alkyne is an excellent building block to develop probes for detecting polyHIS tag proteins via the well-known click chemistry. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tris NTA alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.269 µL	431.347 µL	862.694 µL	4.313 mL	8.627 mL
5 mM	17.254 µL	86.269 µL	172.539 µL	862.694 µL	1.725 mL
10 mM	8.627 µL	43.135 µL	86.269 µL	431.347 µL	862.694 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1159.16 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Differential structural characteristics, physicochemical properties, and calcium-binding capabilities of annexin A2 wild-type versus E53A, E96A, D162A, E247A and D322A mutants.

Authors:

Yoodee, Sunisa and Plumworasawat, Sirikanya and Malaitad, Thanyalak and Peerapen, Paleerath and Thongboonkerd, Visith

Journal:

Archives of biochemistry and biophysics (2025): 110267

Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.

Authors:

Probst, Anna and Knochenhauer, Doreen and Niemeyer, Justus and Fischer, Laura and Schroda, Michael

Journal:

Current genetics (2025): 7

Efficient periplasmic expression of active lysyl endopeptidase and optimizing the purification methods.

Authors:

Pourani, Zahra and Keramati, Malihe and Komijani, Samira and Golkar, Majid and Cohan, Reza Ahangari and Mohseni, Nastaran and Valizadeh, Vahideh

Journal:

Protein expression and purification (2025): 106618

Shotgun and targeted proteomics of Mycolicibacterium smegmatis highlight the role of arginine phosphorylation in the functional adaptation to its environment.

Authors:

Xu, Danyang and Shi, Jiahui and Jiang, Songhao and Meng, Shuhong and Cheng, Zhiyuan and Wu, Wenhui and Chang, Lei and Xie, Yuping and Gao, Yuan and Xue, Yu and Zhang, Yao

Journal:

Journal of proteomics (2025): 105388

Heterologous expression and enzymological characterization of L-glutamate oxidase from the marine actinomycete Streptomyces lydicamycinicus NBRC 110027.

Authors:

Oikawa, Tadao and Yamanaka, Kazuya

Journal:

Bioscience, biotechnology, and biochemistry (2025): 371-381

Physical properties

Molecular weight	1159.16
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 8, 2026