Name: Tris NTA amine
Brand: AAT Bioquest
SKU: 12637
Price: 750 USD
Availability: InStock

Tris NTA amine

Tris-NTA Amine is a polyhistidine-binding reagent with a primary amine for bioconjugation, providing exceptional affinity for capturing and analyzing His-tagged proteins.

Trivalent His-Tag Chelator: Three NTA groups provide nM affinity for His-tagged proteins
Superior Complex Stability: Forms significantly more stable complexes than mono-NTA through multivalent interactions
Primary Amine Reactive Group: Direct conjugation to NHS esters, aldehydes, or isothiocyanates for probe development
Protein Analysis Platform: Facilitates His-tagged protein purification, analysis, and selective labeling in complex biological samples

The chemical structure of Tris NTA amine.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12637	1 mg	Price

Overview

Tris NTA amine is an excellent building block to develop probes for detecting polyHIS tag proteins. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tris NTA amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.324 µL	476.622 µL	953.243 µL	4.766 mL	9.532 mL
5 mM	19.065 µL	95.324 µL	190.649 µL	953.243 µL	1.906 mL
10 mM	9.532 µL	47.662 µL	95.324 µL	476.622 µL	953.243 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1049.05 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Development of an enzymatic aptasensor for monitoring recombinant His-tagged proteins in microbial biotechnology.

Authors:

Jadidi, Mohammad Javad and Emamzadeh, Rahman and Nazari, Mahboobeh and Zaker, Sayed Rasoul

Journal:

Enzyme and microbial technology (2025): 110603

Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture.

Authors:

Aguiar, Tatiana Q and Leal, Tânia and Rodrigues, Diana G and Abrunhosa, Luís and Oliveira, Carla and Domingues, Lucília

Journal:

Talanta (2025): 127126

Ni2+-induced selective precipitation of His-tagged recombinant proteins shortens purification time while maintaining high yield.

Authors:

Islam, Md Din and Islam, M Monirul and Kuroda, Yutaka

Journal:

Journal of biotechnology (2025): 38-46

Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins.

Authors:

Probst, Anna and Knochenhauer, Doreen and Niemeyer, Justus and Fischer, Laura and Schroda, Michael

Journal:

Current genetics (2025): 7

Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character.

Authors:

Yang, Huizhen and Zhao, Ziyan and Li, Hongquan and Wang, Lan

Journal:

Protein expression and purification (2024): 106519

Physical properties

Molecular weight	1049.05
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	862778-60-7

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 16, 2025