Name: Tris NTA maleimide
Brand: AAT Bioquest
SKU: 12634
Price: 765 USD
Availability: InStock

Tris NTA maleimide

Tris-NTA Maleimide selectively conjugates to thiol groups while maintaining high affinity for polyhistidine tags, ideal for developing His-tag-specific detection probes.

Trivalent His-Tag Chelator: Three NTA groups provide nM affinity for His-tagged proteins
High-Stability Protein Binding: Multivalent interaction creates complexes 1000-fold more stable than conventional mono-NTA
Thiol-Selective Chemistry: Maleimide group forms stable thioether bonds with cysteine residues at pH 6.5–7.5
Dual-Function Applications: Combines His-tag affinity purification with site-specific protein conjugation and live-cell labeling

The chemical structure of Tris NTA maleimide.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
12634	1 mg	Price

Overview

Tris NTA malemide is an excellent building block to develop probes for detecting polyHIS tag proteins. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tris NTA maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	83.322 µL	416.608 µL	833.215 µL	4.166 mL	8.332 mL
5 mM	16.664 µL	83.322 µL	166.643 µL	833.215 µL	1.666 mL
10 mM	8.332 µL	41.661 µL	83.322 µL	416.608 µL	833.215 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1200.17 g/mol	=	mL	x	mM	=	mmol

References

View all 9 references: Citation Explorer

Expression and purification of recombinant arginine decarboxylase (speA) from Escherichia coli.

Authors:

Song, Jiaping and Zhou, Chuanwen and Liu, Rui and Wu, Xudong and Wu, Di and Hu, Xiaojian and Ding, Yu

Journal:

Molecular biology reports (2010): 1823-9

[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].

Authors:

Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing'an

Journal:

Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44

Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification.

Authors:

Jonák, J

Journal:

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2007): 141-53

[Displaying polyhistidine peptide on the cell surface of Bacillus thuringiensis by S-layer protein].

Authors:

Wang, Li and Sun, Ming and Yu, Zi Niu

Journal:

Shi yan sheng wu xue bao (2003): 476-81

Lack of antitumor activity of recombinant endostatin in a human neuroblastoma xenograft model.

Authors:

Jouanneau, E and Alberti, L and Nejjari, M and Treilleux, I and Vilgrain, I and Duc, A and Combaret, V and Favrot, M and Leboulch, P and Bachelot, T

Journal:

Journal of neuro-oncology (2001): 11-8

Physical properties

Molecular weight	1200.17
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Room temperature (10-25 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on February 17, 2026