Name: Tris NTA succinimidyl ester
Brand: AAT Bioquest
SKU: 12633
Price: 750 USD
Availability: InStock

Tris NTA succinimidyl ester

Tris-NTA Succinimidyl Ester reacts with primary amines to create high-affinity His-tag binding probes for protein purification and detection applications.

Trivalent His-Tag Chelator: Three NTA groups provide nM affinity for His-tagged proteins
Exceptional Binding Stability: Multivalent design prevents transient dissociation characteristic of mono-NTA systems
Amine-Reactive NHS Chemistry: Stable amide bond formation with lysine residues and N-termini at pH 7-9
Comprehensive Protein Tools: Creates affinity resins, magnetic beads, and fluorescent probes for His-tagged protein purification and detection

The chemical structure of Tris NTA succinimidyl ester.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12633	1 mg	Price

Overview

Tris NTA succinimidyl ester is an excellent building block to develop probes for detecting polyHIS tag proteins. Tris NTA compounds probably have the highest affinity for polyhistidine-labeled proteins. They can be used for the purification and analysis of polyhistidine-tagged proteins. AAT Bioquest also offers a large family of fluorescent tris-NTA compounds that provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA compounds form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tris NTA succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	62.869 µL	314.343 µL	628.686 µL	3.143 mL	6.287 mL
5 mM	12.574 µL	62.869 µL	125.737 µL	628.686 µL	1.257 mL
10 mM	6.287 µL	31.434 µL	62.869 µL	314.343 µL	628.686 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1590.62 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

Engineering Protein Nanoparticles Functionalized with an Immunodominant Coxiella burnetii Antigen to Generate a Q Fever Vaccine.

Authors:

Ramirez, Aaron and Felgner, Jiin and Jain, Aarti and Jan, Sharon and Albin, Tyler J and Badten, Alexander J and Gregory, Anthony E and Nakajima, Rie and Jasinskas, Algimantas and Felgner, Philip L and Burkhardt, Amanda M and Davies, D Huw and Wang, Szu-Wen

Journal:

Bioconjugate chemistry (2023): 1653-1666

Protein Nanoparticle-Mediated Delivery of Recombinant Influenza Hemagglutinin Enhances Immunogenicity and Breadth of the Antibody Response.

Authors:

Badten, Alexander J and Ramirez, Aaron and Hernandez-Davies, Jenny E and Albin, Tyler J and Jain, Aarti and Nakajima, Rie and Felgner, Jiin and Davies, D Huw and Wang, Szu-Wen

Journal:

ACS infectious diseases (2023): 239-252

A Four-Channel Surface Plasmon Resonance Sensor Functionalized Online for Simultaneous Detections of Anti-SARS-CoV-2 Antibody, Free Viral Particles, and Neutralized Viral Particles.

Authors:

Dong, Tianbao and Han, Chaowei and Jiang, Meng and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng

Journal:

ACS sensors (2022): 3560-3570

Regenerable and high-throughput surface plasmon resonance assay for rapid screening of anti-SARS-CoV-2 antibody in serum samples.

Authors:

Jiang, Meng and Dong, Tianbao and Han, Chaowei and Liu, Luyao and Zhang, Tiantian and Kang, Qing and Wang, Pengcheng and Zhou, Feimeng

Journal:

Analytica chimica acta (2022): 339830

Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule-Associated Intracellular Function.

Authors:

Ghosh, Satyajit and Garg, Shubham and Mukherjee, Nabanita and Ghosh, Surajit

Journal:

Methods in molecular biology (Clifton, N.J.) (2022): 17-45

Physical properties

Molecular weight	1590.62
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 15, 2025