Trypan Blue | AAT Bioquest

Trypan Blue, sodium salt

CAS 72-57-1

Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue. Live cells or tissues with intact cell membranes are not be colored. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue color under a microscope. Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells. It is also useful to observe hyphea of fungi and Stramenopiles.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2450	100 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Trypan Blue, sodium salt *CAS 72-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.079 µL	520.394 µL	1.041 mL	5.204 mL	10.408 mL
5 mM	20.816 µL	104.079 µL	208.158 µL	1.041 mL	2.082 mL
10 mM	10.408 µL	52.039 µL	104.079 µL	520.394 µL	1.041 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	960.81 g/mol	=	mL	x	mM	=	mmol

References

View all 106 references: Citation Explorer

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract

Authors:

Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.

Journal:

J Cataract Refract Surg (2006): 1483

A prospective study on trypan blue capsule staining under air vs under viscoelastic

Authors:

Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.

Journal:

Eye (2006): 820

Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity

Authors:

Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.

Journal:

Am J Ophthalmol (2006): 754

Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium

Authors:

Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.

Journal:

Graefes Arch Clin Exp Ophthalmol (2005): 917

Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells

Authors:

Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.

Journal:

Invest Ophthalmol Vis Sci (2005): 304

Physical properties

Molecular weight	960.81
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Room temperature (10-25 °C); Minimize light exposure
UNSPSC	12352200
CAS	72-57-1

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Page updated on November 20, 2024