Trypan Blue, sodium salt *CAS 72-57-1*
Ordering information
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Additional ordering information
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Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 960.81 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Room temperature (10-25 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Cell Viability Assays
CAS 72-57-1 | Molecular weight 960.81 |
Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue. Live cells or tissues with intact cell membranes are not be colored. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue color under a microscope. Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells. It is also useful to observe hyphea of fungi and Stramenopiles.
Example protocol
AT A GLANCE
Important notes
It is important to store at room temperature.
It can be used within 6 months from the date of receipt.
Cat. # |
Product Name |
Unit Size |
2450 |
Trypan Blue, sodium salt *Cell culture tested* |
100 g |
2452 |
Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities* |
10 g |
2455 |
Trypan UltraBlue™, sodium salt *0.1 M aqueous solution* |
1 mL |
2456 |
Trypan Red Plus™ *0.1 M aqueous solution* |
10 mL |
2457 |
Trypan Red Plus™ *0.1 M aqueous solution* |
100 mL |
2465 |
Trypan Purple™ *0.1 M aqueous solution* |
10 mL |
2466 |
Trypan Purple™ *0.1 M aqueous solution* |
100 mL |
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Trypan Blue, sodium salt *CAS 72-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 104.079 µL | 520.394 µL | 1.041 mL | 5.204 mL | 10.408 mL |
5 mM | 20.816 µL | 104.079 µL | 208.158 µL | 1.041 mL | 2.082 mL |
10 mM | 10.408 µL | 52.039 µL | 104.079 µL | 520.394 µL | 1.041 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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References
View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132
Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface
Authors: Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702
Authors: Ch, undefined and ra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702
Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938
Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395
Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152
Trypan-blue- and endoillumination-assisted phacoemulsification in eyes with vitreous hemorrhage during combined cataract and vitreous surgery
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338
Authors: Yamamoto N, Ozaki N, Murakami K.
Journal: Ophthalmologica (2005): 338
Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208
Application notes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
Annexin V