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Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities*
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Application notes
Trypan blue, a diazo dye is a vital stain that colors dead tissues or cells blue. Live cells or tissues with intact cell membranes are not be colored. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells are shown as a distinctive blue color under a microscope. Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability. The method cannot distinguish between necrotic and apoptotic cells. It is also useful to observe hyphea of fungi and Stramenopiles. We offer the highest purity of Trypan Blue.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Trypan Blue, sodium salt *UltraPure grade* *Purified to eliminate fluorescent impurities* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 104.079 µL | 520.394 µL | 1.041 mL | 5.204 mL | 10.408 mL |
| 5 mM | 20.816 µL | 104.079 µL | 208.158 µL | 1.041 mL | 2.082 mL |
| 10 mM | 10.408 µL | 52.039 µL | 104.079 µL | 520.394 µL | 1.041 mL |
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Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 106 references: Citation Explorer
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820
Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754
Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917
Trypan blue: effect on retinal pigment epithelial and neurosensory retinal cells
Authors: Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.
Journal: Invest Ophthalmol Vis Sci (2005): 304
Authors: Narayanan R, Kenney MC, Kamjoo S, Trinh TH, Seigel GM, Resende GP, Kuppermann BD.
Journal: Invest Ophthalmol Vis Sci (2005): 304