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Trypan Purple™
0.1 M aqueous solution
Trypan Purple™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Purple™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Purple™ is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Purple™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Purple™ is highly purified, and can be used up to 0.75 mM with minimal cell cytotoxicity. Our recommended concentration is 0.5 mM.
<p>The structure of Trypan Red Plus&trade; (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).</p>
<p>The structure of Trypan Red Plus&trade; (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).</p>
CatalogSize
Price
Quantity
246510 mL
Price
2466100 mL
Price
 
Physical properties

Molecular weight~600
SolventWater
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 7, 2025