Name: Trypan Purple™ *0.1 M aqueous solution*
Brand: AAT Bioquest
SKU: 2465
Availability: InStock

Trypan Purple™

0.1 M aqueous solution

Trypan Purple™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Purple™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Purple™ is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Purple™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8® AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Purple™ is highly purified, and can be used up to 0.75 mM with minimal cell cytotoxicity. Our recommended concentration is 0.5 mM.

<p>The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).</p>

Protocol

COA

Catalog	Size	Price	Quantity
2465	10 mL	Price
2466	100 mL	Price

References

View all 32 references: Citation Explorer

Indocyanine green and trypan blue: intracellular uptake and extracellular binding by human retinal pigment epithelial cells

Authors:

Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.

Journal:

Retina (2007): 375

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract

Authors:

Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.

Journal:

J Cataract Refract Surg (2006): 1483

Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin

Authors:

Li Q, Kato Y, Sai Y, Imai T, Tsuji A.

Journal:

Pharm Res (2005): 842

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor

Authors:

Heinemann A, Ofner M, Amann R, Peskar BA.

Journal:

Pharmacology (2003): 49

Physical properties

Molecular weight	~600
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 15, 2025