Trypan Purple™ *0.1 M aqueous solution*
| Overview |  SDSProtocol |
See also: Cell Signaling, Cell Cytotoxicity, Cellular Processes, Cell Viability, Membrane Potential and Channels, G-Protein-Coupled Receptors (GPCR)
Molecular weight 600 |
Trypan Purple™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Purple™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Purple™ is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Purple™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Purple™ is highly purified, and can be used up to 0.75 mM with minimal cell cytotoxicity. Our recommended concentration is 0.5 mM.
Example protocol
AT A GLANCE
Important
Expiration date is 6 months from the date of receipt.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Trypan Purple™ *0.1 M aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 166.667 µL | 833.333 µL | 1.667 mL | 8.333 mL | 16.667 mL |
| 5 mM | 33.333 µL | 166.667 µL | 333.333 µL | 1.667 mL | 3.333 mL |
| 10 mM | 16.667 µL | 83.333 µL | 166.667 µL | 833.333 µL | 1.667 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
Citations
View all 1 citations: Citation Explorer
Strain-specific loss of formyl peptide receptor 3 in the murine vomeronasal and immune systems
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775
References
View all 32 references: Citation Explorer
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Authors: Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.
Journal: Retina (2007): 375
Authors: Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.
Journal: Retina (2007): 375
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin
Authors: Li Q, Kato Y, Sai Y, Imai T, Tsuji A.
Journal: Pharm Res (2005): 842
Authors: Li Q, Kato Y, Sai Y, Imai T, Tsuji A.
Journal: Pharm Res (2005): 842
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor
Authors: Heinemann A, Ofner M, Amann R, Peskar BA.
Journal: Pharmacology (2003): 49
Authors: Heinemann A, Ofner M, Amann R, Peskar BA.
Journal: Pharmacology (2003): 49
Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A
Authors: Abrahamse SL, Rechkemmer G.
Journal: Pflugers Arch (2001): 529
Authors: Abrahamse SL, Rechkemmer G.
Journal: Pflugers Arch (2001): 529
Simultaneous optical mapping of transmembrane potential and intracellular calcium in myocyte cultures
Authors: Fast VG, Ideker RE.
Journal: J Cardiovasc Electrophysiol (2000): 547
Authors: Fast VG, Ideker RE.
Journal: J Cardiovasc Electrophysiol (2000): 547
Fluorescent indicators give biased estimates of intracellular free calcium change in aggregating platelets: implication for studies with human von Willebrand factor
Authors: Kermode JC, Zheng Q, Cook EP.
Journal: Blood Cells Mol Dis (1996): 238
Authors: Kermode JC, Zheng Q, Cook EP.
Journal: Blood Cells Mol Dis (1996): 238
Probenecid inhibits platelet responses to aggregating agents in vitro and has a synergistic inhibitory effect with penicillin G
Authors: Packham MA, R and ML, Perry DW, Ruben DH, Kinlough-Rathbone RL.
Journal: Thromb Haemost (1996): 239
Authors: Packham MA, R and ML, Perry DW, Ruben DH, Kinlough-Rathbone RL.
Journal: Thromb Haemost (1996): 239
Quantitative analysis of phagocytosis and killing of Cryptococcus neoformans by human peripheral blood mononuclear cells by flow cytometry
Authors: Chaka W, Scharringa J, Verheul AF, Verhoef J, Van Strijp AG, Hoepelman IM.
Journal: Clin Diagn Lab Immunol (1995): 753
Authors: Chaka W, Scharringa J, Verheul AF, Verhoef J, Van Strijp AG, Hoepelman IM.
Journal: Clin Diagn Lab Immunol (1995): 753
Application notes
Fluorimetric detection of ATPase and protein phosphatase activities by monitoring formation of inorganic phosphate
Annexin V
A Non-Radioactive Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes
How to quantitate ATP induced calcium flux inside mammalian cells using fluorescent calcium indicators