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Trypan Purple™ *0.1 M aqueous solution*

The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
Ordering information
Price ()
Catalog Number2465
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight600
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
600
Trypan Purple™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Purple™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Purple™ is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Purple™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Purple™ is highly purified, and can be used up to 0.75 mM with minimal cell cytotoxicity. Our recommended concentration is 0.5 mM.

Example protocol


AT A GLANCE

Important
Expiration date is 6 months from the date of receipt.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Trypan Purple™ *0.1 M aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM166.667 µL833.333 µL1.667 mL8.333 mL16.667 mL
5 mM33.333 µL166.667 µL333.333 µL1.667 mL3.333 mL
10 mM16.667 µL83.333 µL166.667 µL833.333 µL1.667 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Citations


View all 1 citations: Citation Explorer
Strain-specific loss of formyl peptide receptor 3 in the murine vomeronasal and immune systems
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775

References


View all 32 references: Citation Explorer
Indocyanine green and trypan blue: intracellular uptake and extracellular binding by human retinal pigment epithelial cells
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Journal: Retina (2007): 375
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin
Authors: Li Q, Kato Y, Sai Y, Imai T, Tsuji A.
Journal: Pharm Res (2005): 842
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor
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Journal: Pharmacology (2003): 49
Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A
Authors: Abrahamse SL, Rechkemmer G.
Journal: Pflugers Arch (2001): 529
Simultaneous optical mapping of transmembrane potential and intracellular calcium in myocyte cultures
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Journal: J Cardiovasc Electrophysiol (2000): 547
Fluorescent indicators give biased estimates of intracellular free calcium change in aggregating platelets: implication for studies with human von Willebrand factor
Authors: Kermode JC, Zheng Q, Cook EP.
Journal: Blood Cells Mol Dis (1996): 238
Probenecid inhibits platelet responses to aggregating agents in vitro and has a synergistic inhibitory effect with penicillin G
Authors: Packham MA, R and ML, Perry DW, Ruben DH, Kinlough-Rathbone RL.
Journal: Thromb Haemost (1996): 239
Quantitative analysis of phagocytosis and killing of Cryptococcus neoformans by human peripheral blood mononuclear cells by flow cytometry
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Journal: Clin Diagn Lab Immunol (1995): 753