Trypan Red Plus™ *0.1 M aqueous solution*

Trypan Red Plus™

0.1 M aqueous solution

Trypan Red Plus™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Red Plus™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Red Plus is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Red Plus™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Red Plus™ is highly purified, and can be used up to 1 mM with minimal cell cytotoxicity. Our recommended concentration is 0.75 mM.

<p>The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).</p>

Protocol

COA

Catalog	Size	Price	Quantity
2456	10 mL	Price
2457	100 mL	Price

References

View all 32 references: Citation Explorer

Indocyanine green and trypan blue: intracellular uptake and extracellular binding by human retinal pigment epithelial cells

Authors:

Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.

Journal:

Retina (2007): 375

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract

Authors:

Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.

Journal:

J Cataract Refract Surg (2006): 1483

Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin

Authors:

Li Q, Kato Y, Sai Y, Imai T, Tsuji A.

Journal:

Pharm Res (2005): 842

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor

Authors:

Heinemann A, Ofner M, Amann R, Peskar BA.

Journal:

Pharmacology (2003): 49

Physical properties

Molecular weight	~600
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025