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Trypan Red Plus™ *0.1 M aqueous solution*

The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
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Physical properties
Molecular weight~600
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
~600
Trypan Red Plus™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Red Plus™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Red Plus is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Red Plus™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Red Plus™ is highly purified, and can be used up to 1 mM with minimal cell cytotoxicity. Our recommended concentration is 0.75 mM.

Example protocol


AT A GLANCE

Important
Expiration date is 6 months from the date of receipt.

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Citations


View all 2 citations: Citation Explorer
Comparative Analyses of Murine and Human Formyl Peptide Receptor 3
Authors: Stempel, Hendrik
Journal: (2017)
Strain-specific loss of formyl peptide receptor 3 in the murine vomeronasal and immune systems
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775

References


View all 32 references: Citation Explorer
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Journal: J Cataract Refract Surg (2006): 1483
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Journal: Pharm Res (2005): 842
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
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Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A
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Journal: Pflugers Arch (2001): 529
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