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Trypan Red Plus™ *0.1 M aqueous solution*
Trypan Red Plus™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Red Plus™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Red Plus is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Red Plus™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Red Plus™ is highly purified, and can be used up to 1 mM with minimal cell cytotoxicity. Our recommended concentration is 0.75 mM.
Example protocol
AT A GLANCE
Important
Expiration date is 6 months from the date of receipt.
Citations
View all 2 citations: Citation Explorer
Comparative Analyses of Murine and Human Formyl Peptide Receptor 3
Authors: Stempel, Hendrik
Journal: (2017)
Authors: Stempel, Hendrik
Journal: (2017)
Strain-specific loss of formyl peptide receptor 3 in the murine vomeronasal and immune systems
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775
References
View all 32 references: Citation Explorer
Indocyanine green and trypan blue: intracellular uptake and extracellular binding by human retinal pigment epithelial cells
Authors: Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.
Journal: Retina (2007): 375
Authors: Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.
Journal: Retina (2007): 375
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin
Authors: Li Q, Kato Y, Sai Y, Imai T, Tsuji A.
Journal: Pharm Res (2005): 842
Authors: Li Q, Kato Y, Sai Y, Imai T, Tsuji A.
Journal: Pharm Res (2005): 842
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor
Authors: Heinemann A, Ofner M, Amann R, Peskar BA.
Journal: Pharmacology (2003): 49
Authors: Heinemann A, Ofner M, Amann R, Peskar BA.
Journal: Pharmacology (2003): 49
Page updated on May 13, 2025
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