Trypan Red Plus™ *0.1 M aqueous solution*
| Overview |  SDSProtocol |
See also: Cell Signaling, Cell Cytotoxicity, Cellular Processes, Cell Viability, Membrane Potential and Channels, G-Protein-Coupled Receptors (GPCR)
Molecular weight 600 |
Trypan Red Plus™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Red Plus™ is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Red Plus is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Red Plus™ can also be used to prevent florescent dyes (such as FDA, rhodamine 123, JC-1, TMRE, TMRM, Indo-1 AM, Fura-2 AM, calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Rhod-4™ AM) from leaking out of cells. It might inhibit the activities of drug-efflux pumps since it contains a probenecid-like moiety as shown below. Compared to probenecid, it is neutral, highly soluble in water, and convenient to use. Its cellular mechanism is still under investigation. Our Trypan Red Plus™ is highly purified, and can be used up to 1 mM with minimal cell cytotoxicity. Our recommended concentration is 0.75 mM.
Example protocol
AT A GLANCE
Important
Expiration date is 6 months from the date of receipt.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Trypan Red Plus™ *0.1 M aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 166.667 µL | 833.333 µL | 1.667 mL | 8.333 mL | 16.667 mL |
| 5 mM | 33.333 µL | 166.667 µL | 333.333 µL | 1.667 mL | 3.333 mL |
| 10 mM | 16.667 µL | 83.333 µL | 166.667 µL | 833.333 µL | 1.667 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. The structure of Trypan Red Plus™ (WSG = Water-Soluble Group; PLM = Probenecid-Like Moiety).
Citations
View all 2 citations: Citation Explorer
Comparative Analyses of Murine and Human Formyl Peptide Receptor 3
Authors: Stempel, Hendrik
Journal: (2017)
Authors: Stempel, Hendrik
Journal: (2017)
Strain-specific loss of formyl peptide receptor 3 in the murine vomeronasal and immune systems
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775
Authors: Stempel, Hendrik and Jung, Martin and Pérez-Gómez, Anabel and Leinders-Zufall, Trese and Zufall, Frank and Bufe, Bernd
Journal: Journal of Biological Chemistry (2016): 9762--9775
References
View all 32 references: Citation Explorer
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Authors: Hirasawa H, Yanagi Y, Tamaki Y, Inoue Y, Kadonosono K.
Journal: Retina (2007): 375
Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
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Journal: J Cataract Refract Surg (2006): 1483
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483
Multidrug resistance-associated protein 1 functions as an efflux pump of xenobiotics in the skin
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Authors: Li Q, Kato Y, Sai Y, Imai T, Tsuji A.
Journal: Pharm Res (2005): 842
Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy
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Journal: Gen Physiol Biophys (2004): 357
Authors: Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: Gen Physiol Biophys (2004): 357
A novel assay to measure the calcium flux in human basophils: effects of chemokines and nerve growth factor
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Journal: Pharmacology (2003): 49
Authors: Heinemann A, Ofner M, Amann R, Peskar BA.
Journal: Pharmacology (2003): 49
Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A
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Journal: Pflugers Arch (2001): 529
Authors: Abrahamse SL, Rechkemmer G.
Journal: Pflugers Arch (2001): 529
Simultaneous optical mapping of transmembrane potential and intracellular calcium in myocyte cultures
Authors: Fast VG, Ideker RE.
Journal: J Cardiovasc Electrophysiol (2000): 547
Authors: Fast VG, Ideker RE.
Journal: J Cardiovasc Electrophysiol (2000): 547
Fluorescent indicators give biased estimates of intracellular free calcium change in aggregating platelets: implication for studies with human von Willebrand factor
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Journal: Blood Cells Mol Dis (1996): 238
Authors: Kermode JC, Zheng Q, Cook EP.
Journal: Blood Cells Mol Dis (1996): 238
Probenecid inhibits platelet responses to aggregating agents in vitro and has a synergistic inhibitory effect with penicillin G
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Journal: Thromb Haemost (1996): 239
Authors: Packham MA, R and ML, Perry DW, Ruben DH, Kinlough-Rathbone RL.
Journal: Thromb Haemost (1996): 239
Quantitative analysis of phagocytosis and killing of Cryptococcus neoformans by human peripheral blood mononuclear cells by flow cytometry
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Journal: Clin Diagn Lab Immunol (1995): 753
Authors: Chaka W, Scharringa J, Verheul AF, Verhoef J, Van Strijp AG, Hoepelman IM.
Journal: Clin Diagn Lab Immunol (1995): 753
Application notes
Fluorimetric detection of ATPase and protein phosphatase activities by monitoring formation of inorganic phosphate
Annexin V
A Non-Radioactive Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes
How to quantitate ATP induced calcium flux inside mammalian cells using fluorescent calcium indicators