Tubulite™ Green is a robust tool for fluorescent visualization of tubulin structures in live cells. This cell-permeable probe enables real-time imaging of tubulin dynamics, bypassing the need for cell fixation and allowing continuous assessment of tubulin polymerization and organization. The probe’s green fluorescence and high cellular permeability facilitate its use in multiplexed imaging applications, including co-staining with RFP and nuclear dyes such as DAPI. Upon cellular entry, Tubulite™ Green is hydrolyzed by intracellular esterases, removing its lipophilic blocking group to produce a charged, membrane-impermeant form that is well-retained within cells. Tubulite™ Green is not generally recommended for studies of dynamic cellular processes, as it may interfere with key cellular functions, including tubulin activity and cell division. Additionally, it is unsuitable for fixed-cell imaging, as fluorescence is lost during cell fixation.