Name: Tyramide Sulfo-NHS Ester [Sulpho SHPP]
Brand: AAT Bioquest
SKU: 11044
Price: 97 USD
Availability: InStock

Tyramide Sulfo-NHS Ester

Sulpho SHPP

An excellent building block for TSA (tyramide) probes
An effective tool for increasing protein iodination
Readily react primary amino groups
Highly water soluble

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
11044	25 mg	Price

Overview

Tyramide Sulfo-NHS Ester is an excellent building block to develop TSA (tyramide) probes. It is also called as Water-Soluble Bolton-Hunter Reagent (Sulfo-SHPP) used for preparing tyrosine-like conjugates for increasing the yield of subsequent iodination. The reagent is ideal for iodinating proteins that do not contain tyrosine residues or tyrosine residues with limited accessibility to iodination. Labeling with Sulfo-SHPP also can help to preserve tyrosine residues that are essential for protein function or immunogenicity. It contains an N-hydroxysuccinimide (NHS) ester reactive group that react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. The sulfonated NHS ester of Sulfo-SHPP provides increased reagent solubility in comparison to the non-sulfonated form of Bolton-Hunter Reagent.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Tyramide Sulfo-NHS Ester [Sulpho SHPP] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	273.755 µL	1.369 mL	2.738 mL	13.688 mL	27.376 mL
5 mM	54.751 µL	273.755 µL	547.51 µL	2.738 mL	5.475 mL
10 mM	27.376 µL	136.878 µL	273.755 µL	1.369 mL	2.738 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	365.29 g/mol	=	mL	x	mM	=	mmol

References

View all 3 references: Citation Explorer

Receptor-mediated endocytosis of retinol-binding protein by liver parenchymal cells: interference by radioactive iodination.

Authors:

Malaba, L and Kindberg, G M and Norum, K R and Berg, T and Blomhoff, R

Journal:

The Biochemical journal (1993): 187-91

A simple method for the radioactive iodination of CD4 molecules.

Authors:

Portolés, P and Rojo, J M and Janeway, C A

Journal:

Journal of immunological methods (1990): 105-9

Sites of direct and indirect halogenation of albumin.

Authors:

Knight, L C and Welch, M J

Journal:

Biochimica et biophysica acta (1978): 185-95

Physical properties

Molecular weight	365.29
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	106827-57-0

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Page updated on June 5, 2026