Wheat Germ Agglutinin (WGA)

Wheat Germ Agglutinin, XFD555 Labeled

XFD555, manufactured by AAT Bioquest, has a similar chemical structure to Alexa Fluor® 555, a trademark of Thermo Fisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives, and conjugates are widely used to label yeast bud scars, fibrotic scar tissue, and the cell membrane of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD555 WGA conjugate, like its counterpart Alexa Fluor® 555 WGA conjugate, emits a bright orange fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25503	1 mg	Price

References

View all 50 references: Citation Explorer

Evaluation of human platelet granules by structured illumination laser fluorescence microscopy.

Authors:

Pluthero, Fred G and Kahr, Walter H A

Journal:

Platelets (2023): 2157808

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm.

Authors:

Golub, Oggie and Kilgore, Jason A and Dolman, Nick J

Journal:

Current protocols (2023): e754

Vascular Endothelial Glycocalyx Plays a Role in the Obesity Paradox According to Intravital Observation.

Authors:

Mitsuda, Shingo and Uzawa, Kohji and Sawa, Marie and Ando, Tadao and Yoshikawa, Takahiro and Miyao, Hideki and Yorozu, Tomoko and Ushiyama, Akira

Journal:

Frontiers in cardiovascular medicine (2021): 727888

Loss of troponin-T labelling in endomyocardial biopsies of cardiac transplant patients is associated with increased rejection grading.

Authors:

Isaacson, Mikaiah and Gibbs, Helen and Ruygrok, Peter N and Crossman, David J

Journal:

Clinical and experimental pharmacology & physiology (2020)

Fluorescence background quenching as a means to increase Signal to Background ratio - a proof of concept during Nerve Imaging.

Authors:

Buckle, Tessa and van der Wal, Steffen and van Willigen, Danny M and Aalderink, Germaine and KleinJan, Gijs H and van Leeuwen, Fijs W B

Journal:

Theranostics (2020): 9890-9898

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.08
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	553
Emission (nm)	568
Quantum yield	0.1 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 8, 2024