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Wheat Germ Agglutinin, XFD594 Labeled
XFD594 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 594 (Alexa Fluor® is the trademark of Thermo Fisher). Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. XFD594 conjugate of WGA is equivalent to the Alexa Fluor® 594 conjugate of WGA. It exhibits bright, red fluorescence. XFD594 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues.
Live HeLa cells were stained with Wheat Germ Agglutinin, XFD594 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.
Live HeLa cells were stained with Wheat Germ Agglutinin, XFD594 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using Cy3/TRITC and DAPI filter set.
CatalogSize
Price
Quantity
255091 mg
Price
 
Physical properties

SolventWater
Spectral properties

Absorbance (nm)592
Correction factor (260 nm)0.43
Correction factor (280 nm)0.56
Extinction coefficient (cm -1 M -1)
90000
Excitation (nm)590
Emission (nm)618
Quantum yield
0.66
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings

Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024