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iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate
Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The iFluor® 750 labeled WGA exhibits a distinct far-red fluorescence characteristic, emitting maximally at 779 nm. Notably, the iFluor® 750 WGA conjugate retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.
Live and fixed HeLa cells were stained with iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate at 10 µg/mL for 30 minutes. The image was acquired on a fluorescence microscope using a Cy7 LP filter set.
Live and fixed HeLa cells were stained with iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate at 10 µg/mL for 30 minutes. The image was acquired on a fluorescence microscope using a Cy7 LP filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
255621 mg
Price
 
Physical properties
SolventWater
Spectral properties
Correction factor (260 nm)0.044
Correction factor (280 nm)0.039
Correction factor (565 nm)0.0250
Correction factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)
275000
1
Excitation (nm)757
Emission (nm)779
Quantum yield
0.12
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationCy7 filter set
EmissionCy7 filter set
Recommended plateBlack wall/clear bottom
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Page updated on October 8, 2024