WSP-1 *CAS 1352750-34-5*
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Physical properties
Molecular weight | 561.65 |
Solvent | DMSO |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
CAS 1352750-34-5 | Molecular weight 561.65 |
Hydrogen sulfide (H2S) is an important gaseous mediator, like nitric oxide, that has significant effects on the immunological, neurological, cardiovascular and pulmonary systems of mammals. WSP-1 is a reactive disulfide-containing, fluorescent probe designed to detect H2S in biological samples and cells. Through a reaction-based fluorescent turn-on strategy, WSP-1 selectively and rapidly reacts with H2S to generate benzodithiolone and a fluorophore with excitation and emission maxima of 465 and 515 nm, respectively.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of WSP-1 *CAS 1352750-34-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 178.047 µL | 890.234 µL | 1.78 mL | 8.902 mL | 17.805 mL |
5 mM | 35.609 µL | 178.047 µL | 356.094 µL | 1.78 mL | 3.561 mL |
10 mM | 17.805 µL | 89.023 µL | 178.047 µL | 890.234 µL | 1.78 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 | 0.11001 |
DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 | 0.521 |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 | - |
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - | - |
DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 | 0.521 |
DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - | - |
Citations
View all 1 citations: Citation Explorer
Biodegradable calcium sulfide-based nanomodulators for H2S-boosted Ca2+-involved synergistic cascade cancer therapy
Authors: Lin, Chuchu and Huang, Chenyi and Shi, Zhaoqing and Ou, Meitong and Sun, Shengjie and Yu, Mian and Chen, Ting and Yi, Yunfei and Ji, Xiaoyuan and Lv, Feng and others,
Journal: Acta Pharmaceutica Sinica B (2022)
Authors: Lin, Chuchu and Huang, Chenyi and Shi, Zhaoqing and Ou, Meitong and Sun, Shengjie and Yu, Mian and Chen, Ting and Yi, Yunfei and Ji, Xiaoyuan and Lv, Feng and others,
Journal: Acta Pharmaceutica Sinica B (2022)
References
View all 2 references: Citation Explorer
In site bioimaging of hydrogen sulfide uncovers its pivotal role in regulating nitric oxide-induced lateral root formation
Authors: Li YJ, Chen J, Xian M, Zhou LG, Han FX, Gan LJ, Shi ZQ.
Journal: PLoS One (2014): e90340
Authors: Li YJ, Chen J, Xian M, Zhou LG, Han FX, Gan LJ, Shi ZQ.
Journal: PLoS One (2014): e90340
Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa
Authors: Chen Y, Mo HZ, Zheng MY, Xian M, Qi ZQ, Li YQ, Hu LB, Chen J, Yang LF.
Journal: PLoS One (2014): e110904
Authors: Chen Y, Mo HZ, Zheng MY, Xian M, Qi ZQ, Li YQ, Hu LB, Chen J, Yang LF.
Journal: PLoS One (2014): e110904
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
FAQ
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What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
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How should I reconstitute an NADPH standard?
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
What is the best way to stain cells with Membrane Marker 1-43 (MM 1-43)?