Name: XAF Black™ Ultra Lipofuscin Autofluorescence Blocker
Brand: AAT Bioquest
SKU: 2468
Price: 203 USD
Availability: InStock

XAF Black™ Ultra Lipofuscin Autofluorescence Blocker

XAF Black™ Ultra Lipofuscin Autofluorescence Blocker effectively suppresses lipofuscin and other tissue autofluorescence with superior water solubility and enhanced quenching capability.

High Water Solubility: Enhanced formulation for aqueous applications
Lipofuscin-Specific Blocking: Optimized to suppress autofluorescence from lipofuscin
Superior Quenching Efficiency: More effective than competing products for histochemical staining
Broad Spectrum Suppression: Also reduces background from collagen, elastin, and red blood cells

XAF Black™ Ultra Lipofuscin Autofluorescence Blocker effectively diminishes non-lipofuscin autofluorescence in human lung adenocarcinoma tissue sections across the FITC, Cy®3, and Cy®5 fluorescence channels. Imaging was performed using consistent microscope settings across all channels for both control (untreated) and treated tissue sections. Top panel: Pre-treatment; Bottom panel: Post-treatment.

Catalog	Size	Price	Quantity
2468	1 mL	Price

Overview

XAF Black™ Ultra Lipofuscin Autofluorescence Blocker is a recent upgrade of our outstanding XAF Black™ Lipofuscin Autofluorescence Blocker. It has significantly enhanced quenching capability and greatly improved water solubility. It is specifically formulated to reduce autofluorescence from lipofuscin. XAF Black™ Ultra Lipofuscin Autofluorescence Blocker can be much more effectively used to block the autofluorescence of lipofuscin than other similar products on the market. It can also be used to reduce other background fluorescence, as well as autofluorescence from other sources such as collagen, elastin, and red blood cells. It is a material that can accumulate in aged human and animal tissues. Lipofuscin is a heterogeneous amalgam mainly composed of oxidized proteins (30 to 70%) and lipids such as triglycerides, free fatty acids, cholesterol, and lipoproteins (20 to 50%). Carbohydrates make a small contribution that proportionally may increase with age (4 to 7%). It is generally considered that the protein content has a significant contribution from mitochondria, e.g., ATP synthase subunit residues in congenital ceroid lipofuscinoses. Lipofuscin accumulates in the lysosomes of many cell types with age and/or in patients with severe malnutrition and cancer cachexia. Due to its broad excitation and emission spectra (400 to 700 nm) the presence of lipofuscin complicates the fluorescence imaging of tissues employing exogenous detection fluorophores. The spectrum of lipofuscin overlaps with those of almost all the commonly used detection fluorophores, making it difficult or even impossible to distinguish between specific labelling and autofluorescence caused by lipofuscin. It often hampers fluorescence-based techniques if not properly addressed and corrected for. Autofluorescence is the natural emission of biological substances such as NAD(P)H in liver and vitamin A in hepatic stellate cells. Several other endogenous fluorophores are also known to cause autofluorescence in many tissues.

References

View all 50 references: Citation Explorer

Highlighting the effects of high-intensity interval training on the changes associated with hypertrophy, apoptosis, and histological proteins of the heart of old rats with type 2 diabetes.

Authors:

Rami, Mohammad and Ahmadi Hekmatikar, Amirhossein and Rahdar, Samaneh and Marashi, Sayed Shafa and Daud, D Maryama Awang

Journal:

Scientific reports (2024): 7133

Podocyte injury at young age causes premature senescence and worsens glomerular aging.

Authors:

Veloso Pereira, Beatriz Maria and Zeng, Yuting and Maggiore, Joseph C and Schweickart, Robert Allen and Eng, Diana G and Kaverina, Natalya and McKinzie, Sierra R and Chang, Anthony and Loretz, Carol J and Thieme, Karina and Hukriede, Neil A and Pippin, Jeffrey W and Wessely, Oliver and Shankland, Stuart J

Journal:

American journal of physiology. Renal physiology (2024): F120-F134

Exploring the Effects of Lignin Nanoparticles in Different Zebrafish Inflammatory Models.

Authors:

Bragato, Cinzia and Persico, Andrea and Ferreres, Guillem and Tzanov, Tzanko and Mantecca, Paride

Journal:

International journal of nanomedicine (2024): 7731-7750

Exploring Bacillus amyloliquefaciens strain OM81 for the production of polyhydroxyalkanoate (PHA) bioplastic using olive mill wastewater.

Authors:

Bacha, Samar and Arous, Fatma and Chouikh, Emna and Jaouani, Atef and Gtari, Maher and Charradi, Khaled and Attia, Hamadi and Ghorbel, Dorra

Journal:

3 Biotech (2023): 415

Histological and Histochemical Microscopy Used to Verify 2D-DIGE Pathoproteomics.

Authors:

Zweyer, Margit and Ohlendieck, Kay and Swandulla, Dieter

Journal:

Methods in molecular biology (Clifton, N.J.) (2023): 465-480

Physical properties

Molecular weight	903.22
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 6, 2026