XFD350 amine
XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.
XFD350 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.30 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.43 | 0.56 |
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350* | 343 | 441 | 19000 | 0.25 | 0.19 |
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | 0.25 | 0.19 |
XFD532 amine | 534 | 553 | 81000 | 0.24 | 0.09 |
XFD555 amine | 553 | 568 | 155000 | 0.08 | 0.08 |
XFD568 amine | 579 | 603 | 91300 | 0.45 | 0.46 |
XFD647 amine | 650 | 671 | 270000 | - | 0.03 |
XFD750 amine | 752 | 776 | 290000 | - | 0.04 |
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