XFD350 amine

• Ex/Em: 343/441 nm
• Extinction coefficient: 19,000 cm^-1M^-1
• Reactive Group: Amine
• Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Moderate Brightness & pH Stability: Ideal for high-abundance targets and maintains consistent fluorescence across a broad pH range (4–11)
• High Contrast: Minimizes spectral overlap with common fluorophores like fluorescein and XFD488, enhancing multiplexing and signal clarity

XFD350, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor® 350 (Thermo Fisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.

XFD350 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.