XFD350 azide

• Ex/Em: 343/441 nm
• Extinction coefficient: 19,000 cm^-1M^-1
• Reactive Group: Azide
• Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
• Moderate Brightness & pH Stability: Ideal for high-abundance targets and maintains consistent fluorescence across a broad pH range (4–11)
• High Contrast: Minimizes spectral overlap with common fluorophores like fluorescein and XFD488, enhancing multiplexing and signal clarity

XFD350, manufactured by AAT Bioquest, is structurally similar to Alexa Fluor® 350 (Thermo Fisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.

The azide derivative of XFD350 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.